U-VUE Biomarker Datasheet - Bcl-2

Target:Bcl-2 alpha
Alternative Name:
Clone:SP66
Isotype:Rabbit IgG
Reactivity:Human
Main cell types:lymphomas, mantle B cells and T cells in lymph node
Subcellular Location:Nuclear and cytoplasmic; staining is present on the membrane of the nucleus and mitochondria
Positive control:Lymph node, tonsil

Bcl-2 is a regulatory protein that suppresses apoptosis through the inhibition of pro-apoptotic proteins Bax and Bak. Since the mitochondrial pathway for apoptosis proceeds when molecules stored between the outer and inner mitochondrial membrane are released into the cellular cytoplasm by mitochondrial outer membrane permeabilization, Bcl-2 is able to regulate cell death by controlling the permeability of mitochondrial membrane. In lymphoid tissues Bcl-2 expression can be visualized on mantle B cells and adjacent T cells, but absent in cells within the germinal center. The overexpression of Bcl-2 can occur in certain cancers such as lymphomas where increased expression is occurring to block or delay the onset of apoptosis.

IHC: Anti-Bcl-2 antibody [SP66] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-Bcl-2 antibody conjugate [SP66] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-Bcl-2 antibody conjugate [SP66] staining normal tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Hötzel, Kathy J et al. “Synthetic Antigen Gels as Practical Controls for Standardized and Quantitative Immunohistochemistry.” The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society vol. 67,5 (2019): 309-334. DOI: 10.1369/0022155419832002
  2. Clark Schneider, Kelli M et al. “Dual expression of MYC and BCL2 proteins predicts worse outcomes in diffuse large B-cell lymphoma.” Leukemia & lymphoma vol. 57,7 (2016): 1640-8. DOI: 10.3109/10428194.2015.1101099 
  3. Kendrick, Samantha L et al. “BCL2 antibodies targeted at different epitopes detect varying levels of protein expression and correlate with frequent gene amplification in diffuse large B-cell lymphoma.” Human pathology vol. 45,10 (2014): 2144-53. DOI: 10.1016/j.humpath.2014.06.005

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

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