U-VUE Biomarker Datasheet - CD22

Target:CD22
Alternative Name:Siglec-2
Clone:EPR20061
Isotype:Rabbit IgG
Reactivity:Human
Main cell types:B cells
Subcellular Location:Plasma membrane
Positive control:Tonsil, Spleen

CD22 a transmembrane protein present on the surface of B cells. This receptor belongs to the sialic acid-binding immunoglobulin-like lectin (SIGLEC) family that binds with sialylated glycoproteins, specifically 2, 6-galactose-linked sialic acids that are abundant on the surface of B cells. CD22 plays an immunomodulatory role in B-cell B-cell interactions by inhibiting B-cell antigen receptor-induced signalling. CD22 can also facilitate cell adhesion and cell signaling and is believed to be involved in the localization of B-cells to lymphoid tissues.

IHC: Anti-CD22 antibody [EPR20061] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-CD22 antibody conjugate [EPR20061] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-CD22 antibody conjugate [EPR20061] staining normal tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Griss, Johannes et al. “B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.” Nature communications vol. 10,1 4186. 13 Sep. 2019, DOI: 10.1038/s41467-019-12160-2
  2. Ohtani H, Komeno T, Agatsuma Y, Kobayashi M, Noguchi M, Nakamura N. Follicular Dendritic Cell Meshwork in Angioimmunoblastic T-Cell Lymphoma Is Characterized by Accumulation of CXCL13(+) Cells. J Clin Exp Hematop. 2015;55(2):61-69. DOI: 10.3960/jslrt.55.61
  3. Hoyos, Valentina et al. “Mesenchymal Stromal Cells for Linked Delivery of Oncolytic and Apoptotic Adenoviruses to Non-small-cell Lung Cancers.” Molecular therapy : the journal of the American Society of Gene Therapy vol. 23,9 (2015): 1497-506. DOI: 10.1038/mt.2015.110

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

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