U-VUE Biomarker Datasheet - MHC II

Target:MHC II
Alternative Name:Major histocompatibility complex II
Clone:CR3/43
Isotype:Mouse IgG1
Reactivity:Human
Main cell types:Macrophages, Dendritic cells, B cells, Epithelial cells
Subcellular Location:Membrane
Positive control:Tonsil, Spleen

Major Histocompability Complex II (MHC II) molecules are heterodimer complex that presents peptide antigen on the surface of the professional antigen presenting cells (APC’s) like macrophages and dendritic cells. Presentation of the antigen by MHC II complex is critical in CD4 activation and developement of adaptive immune response. Along with APC’s, B cells and epthelial cells also present the MHC II molecule.

IHC: Anti-MHC II antibody [CR3/43] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-MHC II antibody conjugate [CR3/43] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-MHC II antibody conjugate [CR3/43] staining normal tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Campbell, Michael J et al. “Characterizing the immune microenvironment in high-risk ductal carcinoma in situ of the breast.” Breast cancer research and treatment, vol. 161,1 (2017): 17-28. DOI: 10.1007/s10549-016-4036-0
  2. He, Yayi, et al. “MHC Class II Expression in Lung Cancer.” Lung Cancer, Elsevier, 29 July 2017. DOI: 10.1016/j.lungcan.2017.07.030
  3. Meissner, M, et al. “Loss of Interferon-Gamma Inducibility of the MHC Class II Antigen Processing Pathway in Head and Neck Cancer: Evidence for Post-Transcriptional as Well as Epigenetic Regulation.” The British Journal of Dermatology, U.S. National Library of Medicine, May 2008. DOI: 10.1111/j.1365-2133.2008.08465.x

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

Contact Us