U-VUE Biomarker Datasheet - pan-CK

Target:pan-CK
Alternative Name:pan-Cytokeratin
Clone:AE1/AE3
Isotype:Mouse IgG1
Reactivity:Human
Main cell types:Cells of epithelian origin- including most carcinomas
Subcellular Location:Cytoplasm
Positive control:Tonsil

Cytokeratins play a cytoskeletal role in epithelial tissue and are an important component of intermediate filaments. These provide a structural framework for the cell and help resist mechanical stress. The mixture of AE1 and AE3 clones are able to detect a mixture of low and high molecular weight cytokeratins, thus identifying a broad range of cytokeratins. This marker can be used to identify the epithelial nature of tissue and tumors.

IHC: Anti-pan-CK antibody [AE1/AE3] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-pan-CK antibody conjugate [AE1/AE3] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-pan-CK antibody conjugate [AE1/AE3] staining normal tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Parra ER, Uraoka N, Jiang M, et al. “Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.” Scientific Reports. 2017;7(1):13380. Published 2017 Oct 17. DOI: 10.1038/s41598-017-13942-8
  2. Fankhauser, Christian D et al. “Comprehensive immunohistochemical analysis of PD-L1 shows scarce expression in castration-resistant prostate cancer.” Oncotarget, vol. 9,12 10284-10293. 4 Dec. 2017. DOI: 10.18632/oncotarget.22888
  3. Cossu-Rocca, Paolo, et al. “Acquired Cystic Disease-Associated Renal Tumors: an Immunohistochemical and Fluorescence in Situ Hybridization Study.” Nature News, Modern Pathology, 31 Mar. 2006. DOI: 10.1038/modpathol.3800604

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

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