U-VUE Biomarker Datasheet - PD-L1

Target:PD-L1
Alternative Name:CD274, Programmed cell death ligand 1
Clone:73-10
Isotype:Rabbit IgG
Reactivity:Human
Main cell types:Epithelial, tumor and immune cells
Subcellular Location:Plasma membrane
Positive control:Tonsil

Programmed cell death ligand 1 (PDL1) is a type 1 transmembrane protein (B7-H1) that belongs to the B7 ligands family and may be expressed on both, hematopoietic cells (dendritic cells, macrophages, mast cells, T cells and B cells) and nonhematopoietic cells, including endothelial, epithelial and tumor cells. It plays an immunosuppressive role by inhibiting T-cell activity. Overexpression of PD-L1 by cancer cells may enable them to evade the host immune response, conferring a growth advantage to such tumors.

IHC: Anti-PD-L1 antibody [73-10] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-PD-L1 antibody conjugate [73-10] staining normal tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-PD-L1 antibody conjugate [73-10] staining normal tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Dirix, Luc Y et al. “Avelumab, an anti-PD-L1 antibody, in patients with locally advanced or metastatic breast cancer: a phase 1b JAVELIN Solid Tumor study.” Breast cancer research and treatment, vol. 167,3 (2018): 671-686. DOI: 10.1007/s10549-017-4537-5
  2. Gong, Jun et al. “Development of PD-1 and PD-L1 inhibitors as a form of cancer immunotherapy: a comprehensive review of registration trials and future considerations.” Journal for immunotherapy of cancer, vol. 6,1 8. 23 Jan. 2018. DOI: 10.1186/s40425-018-0316-z
  3. Lalani, Aly-Khan A et al. “Differential expression of c-Met between primary and metastatic sites in clear-cell renal cell carcinoma and its association with PD-L1 expression.” Oncotarget, vol. 8,61 103428-103436. 23 Oct. 2017. DOI: 10.18632/oncotarget.21952

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

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