For Research Use Only, not for use in diagnostic procedures

UltiMapper I/O Immuno8

Get access to 256 distinct binary phenotypes.

Identify and characterize T-cell subsets, immunosuppressive cells, and interactions along the PD-1/PD-L1 checkpoint axis.

This antibody panel consists of the following markers:

  • CD3
  • CD4
  • CD8
  • CD68
  • FoxP3
  • PD-1
  • PD-L1
  • CK/SOX10

 

SKU: Ut-22-5-1 Category:

For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com

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For more information about this product please contact our local distributor in Japan, Cosmo Bio
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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236

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Features

Cell Phenotypes

Cell Phenotyping with the UltiMapper I/O Immuno8 Kit

The antibody panel in the UltiMapper I/O Immuno8 Kit enables users to identify and characterize T-cell subsets, immunosuppressive cells, and interactions along the PD-L1/PD-1 checkpoint axis. CD3 is a marker of T cells. CD4 is phenotypically expressed on the surface of T helper cells. CD8 is a marker for cytotoxic immune cells (primarily cytotoxic T cells). CD68 is a marker of monocytes and macrophages. FoxP3 is a transcription factor and marker of cellular activation. The co-expression of CD3, CD4, and FoxP3 indicated the regulatory T cell (T-reg) phenotype. PD-1 marks exhausted or suppressive T cells (CD3+/PD-1+) and can serve a pro-tumor function to help tumors evade the immune system. PD-L1 is an immune checkpoint marker that can be expressed on both macrophages (CD68+/PD-L1+) and tumor cells (CK/SOX10+/PD-L1). SOX10 is a marker of melanoma tumor cells while pan-cytokeratin detects carcinoma tumor cells.

This multiplex IHC kit allows for the spatial identification of single biomarkers and co-expression of multiple markers in cells enabling the observation of several biologically relevant phenotypes. The above is a partial of the 256 distinct binary phenotypes that this can identify. The number of phenotypes increases if binned marker intensities are taken into account (e.g. PD-1, PD-L1, FoxP3 expression levels).

Markers
PhenotypeCD3CD4CD8CD68FoxP3PD-1PD-L1CK/SOX10
T cells
T helper cells
Cytotoxic immnue cells
Cytotoxic T cells
FoxP3+ T cells
Exhausted T cells
Regulatory T cells(T-reg)
CD4/CD8 Double-positive T cells
CD8+Regulatory T cell (CD8 T-reg)
Exhausted,Cytotoxic T cell
Macrophage
Immunosuppressive macrophage
Tumor cell
Immune-evading tumor cell
Product Biology
MarkerMain Cell TypeFunction
CD3T cellsIdentifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.
CD4Helper T-cellT-helper cells play an important role in the adaptive immune system by helping regulate the immune response. The main cellular phenotype that expresses CD4 is mature helper T cells.
CD8Cytotoxic T cellsCytotoxic T cells are responsible for mediating apoptosis of cancer cells through the release of perforin and granzyme B from the T cells.
CD68MacrophagesMacrophages modulate the immune response.
FoxP3Regulatory T cellFoxP3 is a transcription factor and often regarded as a master regulator of regulatory T cells. Regulatory T cells expressing FoxP3 generally decrease the immune response and can prevent tumor cell infiltration. Recent evidence suggests that FoxP3 is transiently expressed by activated T cells.
PD-1Exhausted T cellA marker of T-cell exhaustion indicating that normal function of the cell has been disrupted. May also indicate an immuno-suppressive phenotype. Increased levels of PD-1+ T cells are associated with a poorer prognosis. PD-1 is the target of multiple therapeutics.
PD-L1Checkpoint proteinAllows cells to escape immune surveillance by binding to PD-1. A search of ClinicalTrials.gov yields nearly 700 clinical trials with the keyword PD-L1. The association of PD-L1 expression with clinical outcome is uncertain at this time, but researchers are working to establish the relationship of this marker to other markers such as CD8.
CK/SOX10Tumor CellsA cocktail of optimized reagents for the detection of pan-cytokeratin and SOX10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.

Frequently Asked Questions

Q How many slides can I stain with a single kit?

A

Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.

Q What image analysis software can I use?

A

Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.

Q What imaging systems do I need to have?

A

UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.

Related Products

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The T cell activation kit enables co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death.

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Characterize regulatory T cells and cytotoxic T cells

The T-reg kit allows you to spatially map the activity and relationships between cytotoxic T cells, regulatory T cells, and T helper cells within the tumor context.

Looking for a Custom Solution?

If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.

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InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

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Services & Biomarker Programs

With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.

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InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

Services

Explore our Services

Standard UltiMapper kits

With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.

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