UltiMapper I/O APC kit staining of professional antigen-presenting cells in a tonsil section.
Ultimapper I/O APC kit staining of professional antigen-presenting cells in a spleen tissue section.
UltiMapper I/O APC kit staining of professional antigen-presenting cells in a tonsil section.

For Research Use Only, not for use in diagnostic procedures

UltiMapper I/O APC

Identify and characterize professional antigen-presenting cells (APCs).

This APC 4-plex/5-color kit enables co-expression to visualize specific subsets of cells along the dendritic, monocyte/macrophage, and B cell lineages.

This antibody panel consists of the following markers:

  • CD11c
  • CD20
  • CD68/CD163
  • MHC Class II
  • Nuclear counterstain
SKU: Ut-22-5 Category:

For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com

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For more information about this product please contact our local distributor in Japan, Cosmo Bio
mail@cosmobio.co.jp, +81 3 5632 9610

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For more information about this product please contact our local distributor in South Korea, MDxK
info@mdxk.co.kr, +82 2 578 8848

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For more information about this product please contact our local distributor in Singapore, AITbiotech
sales@aitbiotech.com, +65 6778 6822

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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
bond.biotech@gmail.com, +886 4 2358 3959

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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236

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Features

Cell Phenotypes

Cell Phenotyping with the UltiMapper™ I/O APC Kit

UltiMapper I/O APC kit staining of professional antigen-presenting cells in a tonsil section.

UltiMapper I/O APC kit (ULT20105) staining tonsil tissue. CD11c (green), CD20 (red), CD68/CD163 (yellow), MHC II (cyan), and nuclear counterstain (blue).

The antibody panel in the UltiMapper I/O APC kit enables users to identify and characterize professional antigen-presenting cells (APCs). CD11c is highly expressed on the surface of dendritic cells. CD20 is a marker of B cells. A cocktail of CD68 and CD163 identifies macrophages. MHC class II is expressed on APCs. The co-localization of these markers allows for the identification of specific subsets of cells along the dendritic, monocyte, macrophage, and B cell lineages.

Markers
PhenotypeCD11cCD20CD68/CD163MHC II
Dendritic cells
B cells
Macrophages
Professional antigen-presenting cells
Product Biology
MarkerMain Cell TypeFunction
CD11cDendritic cellsDendritic cells modulate T cell, B cell, monocyte/macrophage function to link innate and adaptive immunity
CD20B cellsB cells function within the adaptive immune response and are responsible for the development and secretion of antibodies. CD20 is a pan-B cell marker..
CD68/CD163MacrophagesMacrophages modulate the immune response.
MHC IIProfessional antigen-presenting cellsMHC Class II is responsible for the presentation of pathogenic epitopes to the immune system.

Frequently Asked Questions

Q How many slides can I stain with a single kit?

A

Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.

Q What image analysis software can I use?

A

Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.

Q What imaging systems do I need to have?

A

UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.

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