Q How many slides can I stain with a single kit?
Identify and characterize professional antigen-presenting cells (APCs).
This APC 4-plex/5-color kit enables co-expression to visualize specific subsets of cells along the dendritic, monocyte/macrophage, and B cell lineages.
This antibody panel consists of the following markers:
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For more information about this product please contact our local distributor in Japan, Cosmo Bio
mail@cosmobio.co.jp, +81 3 5632 9610
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For more information about this product please contact our local distributor in Singapore, AITbiotech
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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236
UltiMapper I/O APC kit (ULT20105) staining tonsil tissue. CD11c (green), CD20 (red), CD68/CD163 (yellow), MHC II (cyan), and nuclear counterstain (blue).
The antibody panel in the UltiMapper I/O APC kit enables users to identify and characterize professional antigen-presenting cells (APCs). CD11c is highly expressed on the surface of dendritic cells. CD20 is a marker of B cells. A cocktail of CD68 and CD163 identifies macrophages. MHC class II is expressed on APCs. The co-localization of these markers allows for the identification of specific subsets of cells along the dendritic, monocyte, macrophage, and B cell lineages.
Markers | ||||
---|---|---|---|---|
Phenotype | CD11c | CD20 | CD68/CD163 | MHC II |
Dendritic cells | ![]() | |||
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B cells | ![]() | |||
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Macrophages | ![]() | |||
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Professional antigen-presenting cells | ![]() |
Marker | Main Cell Type | Function |
---|---|---|
CD11c | Dendritic cells | Dendritic cells modulate T cell, B cell, monocyte/macrophage function to link innate and adaptive immunity |
CD20 | B cells | B cells function within the adaptive immune response and are responsible for the development and secretion of antibodies. CD20 is a pan-B cell marker.. |
CD68/CD163 | Macrophages | Macrophages modulate the immune response. |
MHC II | Professional antigen-presenting cells | MHC Class II is responsible for the presentation of pathogenic epitopes to the immune system. |
Q How many slides can I stain with a single kit?
Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.
Q What image analysis software can I use?
Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.
Q What imaging systems do I need to have?
UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.
Determine whether the immune response has exhausted
The PD-1 immune exhaustion kit enables co-localization of T cells, memory T cells, tumor cells, and the underlying PD-1 checkpoint immune exhaustion mechanism at play.
Determine whether T cells have the potential to mediate cell death
The T cell activation kit enables co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death.
Characterize regulatory T cells and cytotoxic T cells
The T-reg kit allows you to spatially map the activity and relationships between cytotoxic T cells, regulatory T cells, and T helper cells within the tumor context.
If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.
With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.
With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.