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For Research Use Only, not for use in diagnostic procedures
UltiMapper I/O MDSC
Identify Myeloid Derived Suppressor Cells within the tumor microenvironment
This MDSC 4-plex/5-color kit enables the characterization of myeloid derived suppressor cell populations into M-MDSCs and PMN-MDSCs
This antibody panel consists of the following markers:
- CD11b
- CD14
- CD15
- HLA-DR
- Nuclear counterstain
For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com
For more information about this product please contact our local distributor in Japan, Cosmo Bio
mail@cosmobio.co.jp, +81 3 5632 9610
For more information about this product please contact our local distributor in South Korea, MDxK
info@mdxk.co.kr, +82 2 578 8848
For more information about this product please contact our local distributor in Singapore, AITbiotech
sales@aitbiotech.com, +65 6778 6822
For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
bond.biotech@gmail.com, +886 4 2358 3959
For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236
Questions? Please Contact Us
Features
Cell Phenotypes
Cell Phenotyping with the UltiMapper™ I/O MDSC Kit
UltiMapper I/O MDSC kit staining non-small cell lung cancer tissue. CD11b (green), CD14 (yellow), CD15 (red), HLA-DR (cyan), and nuclear counterstain (blue).
The UltiMapper I/O MDSC Antibody Panel enables users to identify myeloid-derived suppressor cells (MDSCs). MDSCs are a heterogenous population that grow in response to high levels of inflammation and exhibit levels of immune cell suppression. MDSCs exist mainly in two forms: monocytic MDSCs (M-MDSCs) and polymorphonuclear MDSCs (PMN-MDSCs). These cell types can be characterized based on the co-expression of markers.
| Markers | ||||
|---|---|---|---|---|
| Phenotype | CD11b | CD14 | CD15 | HLA-DR |
| Myeloid cell/lymphoid cell |  | |||
| Monocyte/Macrophage/Dendritic cell | | | | |
| M-MDSC | | | (-/low) | |
| Granulocyte (neutrophil/eosinophil) | | | | |
| PMN-MDSC | | | (-/low) | |
| Antigen-presenting cell | | |||
This multiplex IHC kit enables the spatial identification of single biomarkers and co-expression in cells enabling the observation of several phenotypes. The above is a partial list of the 16 distinct binary phenotypes that this kit can identify. The number of phenotypes increases if binned marker intensities are taken into account (e.g. expression of HLA-DR)
Product Biology
| Marker | Main Cell Type | Function |
|---|---|---|
| CD11b | Myeloid cell | CD11b is primarily expressed on cells of a myeloid lineage. Myeloid cells differentiate into specific cell types such as neutrophils, monocytes, macrophages, and dendritic cells.v |
| CD14 | Monocyte | Monocytes are large myeloid cells that can often differentiate into macrophages and dendritic cells. These cells coexpress CD14 and HLA-DR. In the context of MDSCs, M-MDSCs are in a paused state on their path from myeloid to monocytic differentation and express CD14, but lack HLA-DR expression. |
| CD15 | Granulocyte | Granulocytes are a type of myeloid cell that are characterized by the presence of granules in the cytoplasm, and have a varying nuclear shape; often termed as polymorphonuclear (PMN) leukocytes. Granucloytes can differentiate into neutrophils (prominent cell type), eosinophils, basophils, and mast cells. In the context of MDSCs, PMN-MDSCs are in a paused state on their development path from myeloid to neutrophil differentiation and express CD15, but lack or weakly express HLA-DR. |
| HLA-DR | Antigen-presenting cell | Human leukocyte antigen (HLA) complex encodes the major histocompatibility complex (MHC). HLA-DR is the main isotype of 3 isotype (-DR, -DP, -DQ) responsible for presentation of antigens to T cells and B cells. Often, HLA-DR is used as a marker indicating the presence of antigen-presenting cells (APC). |
Frequently Asked Questions
A
Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.
Q What image analysis platforms can I use?
A
Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.
Q What imaging systems do I need to have?
A
UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.
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UltiMapper I/O PD-L1
Determine whether the tumor is “hot” or “cold”
The PD-L1 immune infiltration kit enables co-localization of macrophages, cytotoxic T cells, tumor cells, and the underlying inhibitory or inflammatory mechanisms at play along the PD-L1 axis.
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Determine whether T cells have the potential to mediate cell death
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Looking for a Custom Solution?
If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.
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InSituPlex
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.
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Services & Biomarker Programs
With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.
Explore our Technology
InSituPlex
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.
Explore our Services
Services & Programs
With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.