For Research Use Only, not for use in diagnostic procedures

UltiMapper I/O MDSC

Identify Myeloid Derived Suppressor Cells within the tumor microenvironment

This MDSC 4-plex/5-color kit enables the characterization of myeloid derived suppressor cell populations into M-MDSCs and PMN-MDSCs

This antibody panel consists of the following markers:

  • CD11b
  • CD14
  • CD15
  • HLA-DR
  • Nuclear counterstain
SKU: Ut-22-1 Category:

For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com

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For more information about this product please contact our local distributor in Japan, Cosmo Bio
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For more information about this product please contact our local distributor in Singapore, AITbiotech
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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236

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Features

Cell Phenotypes

Cell Phenotyping with the UltiMapper™ I/O MDSC Kit

UltiMapper I/O MDSC kit staining non-small cell lung cancer tissue. CD11b (green), CD14 (yellow), CD15 (red), HLA-DR (cyan), and nuclear counterstain (blue).

The UltiMapper I/O MDSC Antibody Panel enables users to identify myeloid-derived suppressor cells (MDSCs). MDSCs are a heterogenous population that grow in response to high levels of inflammation and exhibit levels of immune cell suppression. MDSCs exist mainly in two forms: monocytic MDSCs (M-MDSCs) and polymorphonuclear MDSCs (PMN-MDSCs). These cell types can be characterized based on the co-expression of markers.

Markers
PhenotypeCD11bCD14CD15HLA-DR
Myeloid cell/lymphoid cell
Monocyte/Macrophage/Dendritic cell
M-MDSC(-/low)
Granulocyte (neutrophil/eosinophil)
PMN-MDSC(-/low)
Antigen-presenting cell

This multiplex IHC kit enables the spatial identification of single biomarkers and co-expression in cells enabling the observation of several phenotypes. The above is a partial list of the 16 distinct binary phenotypes that this kit can identify. The number of phenotypes increases if binned marker intensities are taken into account (e.g. expression of HLA-DR)

Product Biology
MarkerMain Cell TypeFunction
CD11bMyeloid cellCD11b is primarily expressed on cells of a myeloid lineage. Myeloid cells differentiate into specific cell types such as neutrophils, monocytes, macrophages, and dendritic cells.v
CD14MonocyteMonocytes are large myeloid cells that can often differentiate into macrophages and dendritic cells. These cells coexpress CD14 and HLA-DR. In the context of MDSCs, M-MDSCs are in a paused state on their path from myeloid to monocytic differentation and express CD14, but lack HLA-DR expression.
CD15GranulocyteGranulocytes are a type of myeloid cell that are characterized by the presence of granules in the cytoplasm, and have a varying nuclear shape; often termed as polymorphonuclear (PMN) leukocytes. Granucloytes can differentiate into neutrophils (prominent cell type), eosinophils, basophils, and mast cells. In the context of MDSCs, PMN-MDSCs are in a paused state on their development path from myeloid to neutrophil differentiation and express CD15, but lack or weakly express HLA-DR.
HLA-DRAntigen-presenting cellHuman leukocyte antigen (HLA) complex encodes the major histocompatibility complex (MHC). HLA-DR is the main isotype of 3 isotype (-DR, -DP, -DQ) responsible for presentation of antigens to T cells and B cells. Often, HLA-DR is used as a marker indicating the presence of antigen-presenting cells (APC).

Frequently Asked Questions

Q How many slides can I stain with a single kit?

A

Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.

Q What image analysis platforms can I use?

A

Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.

Q What imaging systems do I need to have?

A

UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.

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Looking for a Custom Solution?

If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.

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InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

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With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.

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Explore our Technology

InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

Services

Explore our Services

Services & Programs

With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.

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