Magnified image of UltiMapper I/O PD-1 kit staining displaying the abundance of memory T cells and T cells expressing the marker PD-1 in tumor tissue.
UltiMapper I/O PD-1 kit staining of non-small cell lung cancer tissue.
Magnified image of UltiMapper I/O PD-1 kit staining displaying the abundance of memory T cells and T cells expressing the marker PD-1 in tumor tissue.
UltiMapper I/O PD-1 kit staining of non-small cell lung cancer tissue.

For Research Use Only, not for use in diagnostic procedures

UltiMapper I/O PD-1

Determine whether the immune response has exhausted.

Our Immune exhaustion 4-plex/5-color kit enables co-localization of T cells, memory T cells, tumor cells, and the underlying PD-1 checkpoint immune exhaustion mechanism at play.

This antibody panel consists of the following markers:

  • CD3
  • CD45RO
  • PD-1
  • panCK / SOX10
  • Nuclear counterstain
SKU: Ut-22-3 Category:

For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com

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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236

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Features

Cell Phenotypes

Cell Phenotyping with the UltiMapper™ I/O PD-1 Kit

UltiMapper I/O PD-1 kit staining of non-small cell lung cancer tissue.

UltiMapper I/O PD-1 kit (ULT20102) staining non-small cell lung cancer tissue. CD3 (green), CD45RO (yellow), PD-1 (red), panCK (cyan), and nuclear counterstain (blue).

The antibody panel in the UltiMapper I/O PD-1 kit enables users to profile tumor samples by detecting the location and abundance of memory T cells and exhausted T cells. CD3 is a general marker for T cells. CD45RO is a marker for memory T cells, which have been primed against antigens and can provide anti-tumor immune responses. PD-1 marks exhausted or suppressive T cells and can serve a pro-tumor function to help tumors evade the immune system. SOX10 is a tumor marker for melanomas while panCK detects carcinomas; antibodies for panCK and SOX10 are provided as a cocktail in this kit.

Markers
PhenotypeCD3CD45ROPD-1panCK/SOX10
T cells
Memory cells
Exhausted cells
Carcinoma or Melanoma
Memory T cells
Exhausted memory cells
Exhausted T cells
Exhausted memory T cells
Product Biology
MarkerMain Cell TypeFunction
CD3T cellsIdentifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.
CD45ROMemory T cellIndicates that the T cell has previously encountered its cognate antigen. An increase in CD45RO+ T cells in tumors is associated with a better prognosis.
PD-1Exhausted T cellA marker of T-cell exhaustion indicating that normal function of the cell has been disrupted. May also indicate an immuno-suppressive phenotype. Increased levels of PD-1+ T cells are associated with a poorer prognosis. PD-1 is the target of multiple therapeutics.
CK/Sox10Tumour cellsThe kit may be used to interrogate many tumor sample types for Research. A cocktail of optimized reagents for the detection of pan-Cytokeratin and Sox10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.

Frequently Asked Questions

Q How many slides can I stain with a single kit?

A

Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.

Q What image analysis platforms can I use?

A

Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.

Q What imaging systems do I need to have?

A

UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.

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The T-reg kit allows you to spatially map the activity and relationships between cytotoxic T cells, regulatory T cells, and T helper cells within the tumor context.

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Determine whether the tumor is “hot” or “cold”

This PD-L1 immune infiltration kit enables co-localization of macrophages, cytotoxic T cells, tumor cells, and the underlying inhibitory or inflammatory mechanisms at play along the PD-L1 axis.

Looking for a Custom Solution?

If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.

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InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

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Services & Biomarker Programs

With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.

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Explore our Technology

InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

Services

Explore our Services

Services & Programs

With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.

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