Q How many slides can I stain with a single kit?
Determine whether the tumor is “hot” or “cold”.
Our Immune infiltration 4-plex/5-color kit enables co-localization of macrophages, cytotoxic T cells, tumor cells, and the underlying inhibitory or inflammatory mechanisms at play along the PD-L1 axis.
This antibody panel consists of the following markers:
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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236
UltiMapper I/O PD-L1 kit (ULT20101) staining non-small cell lung cancer tissue. CD8 (green), CD68 (yellow), PD-L1 (red), panCK (cyan), and nuclear counterstain (blue).
The antibody panel in the UltiMapper I/O PD-L1 kit enables users to detect immune infiltration and profile tumor samples. CD8 is a marker for cytotoxic immune cells (mainly cytotoxic T cells). CD68 is a marker for macrophages. PD-L1 is an immune checkpoint marker that can be expressed on both macrophages and tumor cells. SOX10 is a tumor marker for melanomas while panCK detects carcinomas; antibodies for panCK and SOX10 are provided as a cocktail in this kit. The expression level of PD-L1+ tumor cells and their proximity to CD8+ T cells have been highlighted as potential predictors of therapeutic response.
Markers | ||||
---|---|---|---|---|
Phenotype | CD8 | CD68 | PD-L1 | panCK/SOX10 |
Cytotoxic immune cells | ![]() | |||
Macrophages | ![]() | |||
PD-L1 checkpoint expression | ![]() | |||
Carcinoma (panCK) or Melanoma (SOX10) | ![]() | |||
Immunosuppressive macrophages | ![]() | ![]() | ||
Immune evading tumor cells | ![]() | ![]() |
Marker | Main Cell Type | Function |
---|---|---|
CD8 | Cytotoxic T cells | Cytotoxic T-cells are responsible for mediating apoptosis of cancer cells through the release of perforin and granzyme B from the T-cells. Increased levels of PD-L1+ cells alone in tumor samples provide limited differentiation between samples. However, the proximity of PD-L1+ cells to CD8+ cells may support better differentiation. |
CD68 | Macrophages | Macrophages modulate the immune response. |
PD-L1 | Checkpoint protein | Allows cells to escape immune surveillance by binding to PD-1. A search of ClinicalTrials.gov yields nearly 700 clinical trials with the keyword PD-L1. The association of PD-L1 expression with clinical outcome is uncertain at this time, but researchers are working to establish the relationship of this marker to other markers such as CD8 |
CK/Sox10 | Tumor cells | A cocktail of optimized reagents for the detection of pan-Cytokeratin and Sox10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas. |
Q How many slides can I stain with a single kit?
Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.
Q What image analysis platforms can I use?
Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.
Q What imaging systems do I need to have?
UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.
Determine whether the immune response has exhausted.
The PD-1 immune exhaustion kit enables co-localization of T cells, memory T cells, tumor cells, and the underlying PD-1 checkpoint immune exhaustion mechanism at play.
Identify and characterize professional antigen-presenting cells (APCs).
The APC kit enables co-expression to visualize specific subsets of cells along the dendritic, monocyte/macrophage, and B cell lineages.
Characterize regulatory T cells and cytotoxic T cells
The T-reg kit allows you to spatially map the activity and relationships between cytotoxic T cells, regulatory T cells, and T helper cells within the tumor context.
If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications.
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation
With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.
Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation
With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.