Gaining key phenotypic data from the tumor microenvironment using 8-plex immunofluorescence staining and image analysis
Angela Vasaturo, PhD; Senior Scientist, Ultivue
Lorcan Sherry, PhD; Chief Science Officer, OracleBio
Broadcast via The Pathologist, November 2020. Multiplex staining and image analysis are being increasingly deployed for immuno-oncology (I/O) evaluations to investigate and unravel the complexity of immune cell/tumor cell interactions in the tumor microenvironment (TME) and in discovering novel predictive biomarkers for targeted immunotherapy. Immune checkpoint inhibitor treatment, guided by PD-L1 and PD-1 tissue expression assessed by immunohistochemistry, has already shown to improve outcomes for non-small cell lung carcinoma (NSCLC) and colorectal cancer (CRC) metastatic patients. During this webinar, we’ll present a workflow utilizing an 8-plex staining protocol and detailed image analysis approach for the comprehensive assessment of the immune cell types and interactions within the TME, including co-localization and spatial phenotyping analysis in NSCLC and CRC tumor types.
- A fast, optimized and validated 8-plex multiplex immunofluorescence staining protocol and a comprehensive image analysis can be applied to a broad range of cancer types in order to characterize the immune contexture.
- The ability to carry out multiparameter cellular analysis facilitates the delivery of key cell phenotypes as well as their functional status, thereby leading to greater understanding of tumor biology.