U-VUE Biomarker Datasheet - CD4

Target:CD4
Alternative Name:T-cell surface antigen T4
Clone:SP35
Isotype:Rabbit IgG
Reactivity:Human
Main cell types:T helper cell
Subcellular Location:Plasma membrane
Positive control:Tonsil, Spleen

CD4 is expressed by thymocytes, helper T cells, dendritic cells, type II NKT cells, and monocytes/macrophages. CD4 is a member of the immunoglobulin superfamily and is part of the TCR/CD3 complex, binding to MHC class II molecules and participating in signal transduction through recruitment of tyrosine kinase Lck. CD4 expression is used to identify helper T cells of which there are many different subsets including Th1, Th2, Th9, Th17, regulatory T cell, follicular helper T cell, each contributing to immune function through their unique cytokine profile.

IHC: Anti-CD4 antibody [SP35] staining human tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

IHC: Anti-CD4 antibody conjugate [SP35] staining human tonsil FFPE tissue (brown). Hematoxylin counterstain in blue.

ISP: Anti-CD4 antibody conjugate [SP35] staining human tonsil FFPE tissue (green). Nuclear counterstain in blue.

References

  1. Halse, H et al. “Multiplex immunohistochemistry accurately defines the immune context of metastatic melanoma.” Scientific reports, vol. 8,1 11158. 24 Jul. 2018. DOI: 10.1038/s41598-018-28944-3
  2. Parra, Edwin R et al. “Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.” Scientific reports, vol. 7,1 13380. 17 Oct. 2017. DOI: 10.1038/s41598-017-13942-8
  3. Sade-Feldman, Moshe et al. “Resistance to checkpoint blockade therapy through inactivation of antigen presentation.” Nature communications, vol. 8,1 1136. 26 Oct. 2017. DOI: 10.1038/s41467-017-01062-w

Verification

Each lot of antibody conjugate reagent is quality control tested on positive control tissue and reviewed by Ultivue’s Pathology and Biomarker Analytics group to ensure appropriate staining patterns and signal intensity.

This U-VUE Antibody Conjugate is for Research Use Only. Not for Diagnostic purposes.

Contact Us