Expand your insights into the TME (tumor microenvironment):
Assay services with up to 12-plex OmniVUE™ and U-VUE® panels
Generate more insights from a single slide by visualizing up to 12 biomarkers simultaneously. InSituPlex® assays provide unprecedented consistency and high dynamic range.
Benefits of 12-plex panels
More phenotypes
Detect additional complex phenotype matrices from a single tissue sample, using AI to distinguish cells in challenging, low signal-to-background regions.
Deeper understanding
Gain a deeper understanding of the tissue microenviroment by exploring vasculature, T cells, B cells and other tumor biomarkers in a single panel.
More insights, from fewer slides
Get more insights from precious tissue specimen such as rare tissues, small biopsy samples, and more.
More biomarkers, greater insights from a single tissue sample
Gain deeper phenotypic insight from critical disease markers below.
Design your OmniVUE™ Panel
Guide: To create your panel, select up to 8 markers from our Core Biomarker Library on the left. Simply drag and drop the markers onto the custom panel configurator on the right. Descriptions of all markers are shown below. When you have your panel ready, give it a name and click on Request Panel (minimum order size of 5 kits).
Results
My OmniVUE™ panel
Select up to 8 markers from the list from the left and drag and drop them here.
Explore our Core Biomarker Library
Marker descriptions
CD11b
CD11b is an integrin alpha M chain protein and is expressed by myeloid derived cells. It plays a key role in adherence of leukocytes to stimulate endothelium and mediates uptake of the complementcoated particles. Recent studies identify CD11b as a negative regulator of immune suppression and a target for cancer immune therapy. (Download Biomarker Datasheet)
CD11c
CD11c is one of four β2 integrins along with CD11a, CD11b and CD11d. CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). It is a receptor for fibrinogen and functions in chemotaxis and cell adhesion. Integrins mediate myeloid cell recruitment from the blood vessels into tissue and lymph nodes and contribute to the immunological synapse between T cells and antigen presenting cells. (Download Biomarker Datasheet)
CD14
CD14 is a pattern recognition receptor that detects the pathogen-associate molecular patterns found on the surface of microorganisms. It is a co-receptor located on the cell surface that mediates the innate immune response. (Download Biomarker Datasheet)
CD15
CD15 is a cluster of cell surface glycoproteins and glycolipids, also known as 3-fucosyl-N-acetyl-lactosamine or Lewis X. CD15 is a carbohydrate adhesion molecule that functions in cell-to-cell recognition processes. It is a distinguishing marker for human myeloid cells and mediates neutrophil adhesion to dendritic cells. Several studies have shown that CD15 expression is associated with prognosis and survival in a variety of cancers, such as breast cancer and Hodgkin’s lymphoma. (Download Biomarker Datasheet)
CD163
CD163 is a type I transmembrane protein belonging to the group B of the scavenger receptor cysteine-rich superfamily. It is involved in the clearance and endocytosis of haptoglobin-hemoglobin complexes and has been widely used to identify M2 type macrophage. The scavenging role of CD163 is critical to its anti-inflammatory response, and recent findings have shown the significance of CD163-positive macrophages in tumor progression. (Download Biomarker Datasheet)
CD20
CD20 is a B cell differentiation antigen expressed in B cell development from early pre-B cell stage to mature B cell stage but lost on differentiation into plasma cells. Its role is in regulating B- cell activation, proliferation and differentiation. Aberrant CD20 expression has been described in mainly B cell tumor types such as Burkitt lymphoma, non-Hodgkin lymphoma, and chronic lymphocytic leukemia (CLL). (Download Biomarker Datasheet)
CD206
CD206 is a C-type lectin that can be found on certain populations of macrophages and dendritic cells. Also known as mannose receptor C type 1 (MRC1), it is normally expressed on M2 macrophages. CD206 is thought to play a role in innate and adaptive immunity by acting as a pattern recognition receptor for various pathogens. (Biomarker Datasheet coming soon)
CD3
CD3 is a multimeric protein composed of 4 subunits (γ, δ, ε, ζ), which are part of the T-cell receptor (TCR). Engagement of CD3 induces downstream signaling events that result in T-cell activation. The specificity of the CD3 antigen for T cells and its appearance at all stages of T cell development makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms. (Download Biomarker Datasheet)
CD4
CD4 is a member of the immunoglobulin superfamily and is part of the TCR/CD3 complex, binding to MHC class II molecules and participating in signal transduction through recruitment of tyrosine kinase Lck. CD4 expression is used to identify helper T cells of which there are many different subsets including Th1, Th2, Th9, Th17, regulatory T cell, and follicular helper T cell. Each of these contributes to immune function through their unique cytokine profile. (Download Biomarker Datasheet)
CD45RO
CD45RO is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. Lack of CD45RO on T cells indicates naive T cell subsets while CD45RO expression indicated previous antigen exposure and defines the memory T cell subset. High density of CD45RO+ T cells in solid tumors is associated with a better prognosis. (Download Biomarker Datasheet)
CD56
CD56, also known as neural cell adhesion molecule (NCAM) is a transmembrane glycoprotein often considered a marker of neural lineage commitment due to its discovery site. CD56 expression is most strongly associated with natural killer (NK) cells but it has also been detected on other lymphoid cells, including gamma delta (γδ), T cells and activated CD8+ T cells, as well as dendritic cells. (Download Biomarker Datasheet)
CD68
CD68 is expressed on human macrophages and other mononuclear phagocytes. CD68 is a heavily glycosylated glycoprotein that is involved in ligand binding and is a member of the scavenger receptor family. CD68 functions in phagocytic activities and macrophage homing. An increased CD68+ macrophage index is associated with metastasis, shorter disease-free interval, poor prognosis, and reduced overall survival in multiple types of cancer. (Download Biomarker Datasheet)
CD8
CD8 is primarily expressed on cytotoxic T cells, but it can also be expressed on cortical thymocytes, dendritic cells and NK cells. CD8 is a transmembrane glycoprotein that is a co-receptor for the T cell receptor (TCR). CD8 binds MHC Class I to aid in antigen recognition and TCR-mediated activation. CD8 forms dimers of CD8ɑ and CD8β and clone C8/144B recognizes the alpha form of CD8. (Download Biomarker Datasheet)
CK
Cytokeratins play a cytoskeletal role in epithelial tissue and are an important component of intermediate filaments. These provide a structural framework for the cell and help resist mechanical stress. The mixture of AE1 and AE3 clones are able to detect a mixture of low and high molecular weight cytokeratins, thus identifying a broad range of cytokeratins. This marker can be used to identify the epithelial nature of tissue and tumors. (Download Biomarker Datasheet)
FoxP3
FoxP3, or Forkhead Box P3 is a transcription factor important in the development and inhibitory function of regulatory T cells (Tregs). FoxP3 functions by inhibiting cytokine production and T cell effector function, thus playing a crucial role in maintenance of immunological tolerance and control of immune responses against tumors and pathogens. (Download Biomarker Datasheet)
GrzB
Granzyme B (GrzB) is a serine protease stored in secretory granules of Cytotoxic T lymphocytes (CTLs) and Natural killer (NK) cells. Activated cytotoxic cells release granzyme B which enters the target cells where it can interact with cellular substrates to initiate cell death. (Download Biomarker Datasheet)
HLA-DR
Human leukocyte antigen (HLA) complex encodes the major histocompatibility complex (MHC). HLA-DR is the main isotype of 3 isotype (-DR, -DP, -DQ) responsible for presentation of antigens to T cells and B cells. Often, HLA-DR is used as a marker indicating the presence of antigen-presenting cells. HLA-DR expression in tumors has been shown to be positively associated with patient prognosis in some cancers such as colorectal cancer but is negatively associated with prognosis in other cancer types, such as glioma. (Download Biomarker Datasheet)
Ki67
Ki67 is a nuclear marker associated with cellular proliferation. Ki67 is present within the nucleus of cells undergoing division during interphase but is absent in quiescent cells. Ki67 can also be used as a prognostic indicator in certain cancers. (Download Biomarker Datasheet)
MHC II
Major Histocompability Complex II (MHC II) molecules are heterodimer complex that presents peptide antigen on the surface of the professional antigen presenting cells (APC’s) like macrophages and dendritic cells. Presentation of the antigen by MHC II complex is critical in CD4 activation and development of adaptive immune response. Along with APC’s, B cells and epithelial cells also present the MHC II molecule. (Biomarker Datasheet coming soon)
PD1
Programmed cell death protein 1, also known as PD1 and CD279, is an inhibitory receptor expressed by all T cells during activation. It regulates T cell effector functions during physiological responses, including acute and chronic infection, cancer, and autoimmunity. When PD-1 is bound to his ligand PD-L1, it prevents T cells from killing target cells including cancer cells. (Download Biomarker Datasheet)
PD-L1
Programmed cell death ligand 1 (PD-L1) is a type 1 transmembrane protein (B7-H1) that belongs to the B7 ligands family and may be expressed on both, hematopoietic cells (dendritic cells, macrophages, mast cells, T cells and B cells) and non-hematopoietic cells, including endothelial, epithelial and tumor cells. It plays an immunosuppressive role by inhibiting T-cell activity. Overexpression of PD-L1 by cancer cells may enable them to evade the host immune response, conferring a growth advantage to such tumors. (Download Biomarker Datasheet)
Sox10
Sox10 (Sry-related HMg-Box gene 10) is a nuclear transcription factor invovled in differentiation of neural crest progenator cells to melanocytes and maintanace of Schwann cells. Its high expression is observed in the melanocytic tumors of skin, soft tissue and primary as well as metastatic melanoma. (Download Biomarker Datasheet)
CK/Sox10
Cytokeratins play a cytoskeletal role in epithelial tissue and are an important component of intermediate filaments. These provide a structural framework for the cell and help resist mechanical stress. The mixture of AE1 and AE3clones can detect a mixture of low and high molecular weight cytokeratins, thus identifying a broad range of cytokeratins. This marker can be used to identify the epithelial nature of tissue and tumors. (Download Biomarker Datasheet)
SOX10 (Sry-related HMg-Box gene 10) is a nuclear transcription factor involved in differentiation of neural crest progenitor cells to melanocytes and maintenance of Schwann cells. Its high expression is observed in the melanocytic tumors of skin, soft tissue and primary as well as metastatic melanoma. (Download Biomarker Datasheet)
CD68/CD163
CD68 is expressed on human macrophages and other mononuclear phagocytes. CD68 is a heavily glycosylated glycoprotein that is involved in ligand binding and is a member of the scavenger receptor family. CD68 functions in phagocytic activities and macrophage homing. An increased CD68+ macrophage index is associated with metastasis, shorter disease-free interval, poor prognosis, and reduced overall survival in multiple types of cancer. (Download Datasheet)
CD163 is a type I transmembrane protein belonging to the group B of the scavenger receptor cysteine-rich superfamily. It is involved in the clearance and endocytosis of haptoglobin-hemoglobin complexes and has been widely used to identify M2 type macrophage. The scavenging role of CD163 is critical to its anti-inflammatory response, and recent findings have shown the significance of CD163-positive macrophages in tumor progression. (Download Biomarker Datasheet)
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Marker descriptionsSelect
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Explore our Core Biomarker Library
Marker descriptions
CD11b
CD11b is an integrin alpha M chain protein and is expressed by myeloid derived cells. It plays a key role in adherence of leukocytes to stimulate endothelium and mediates uptake of the complementcoated particles. Recent studies identify CD11b as a negative regulator of immune suppression and a target for cancer immune therapy. (Download Biomarker Datasheet)
CD11c
CD11c is one of four β2 integrins along with CD11a, CD11b and CD11d. CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). It is a receptor for fibrinogen and functions in chemotaxis and cell adhesion. Integrins mediate myeloid cell recruitment from the blood vessels into tissue and lymph nodes and contribute to the immunological synapse between T cells and antigen presenting cells. (Download Biomarker Datasheet)
CD14
CD14 is a pattern recognition receptor that detects the pathogen-associate molecular patterns found on the surface of microorganisms. It is a co-receptor located on the cell surface that mediates the innate immune response. (Download Biomarker Datasheet)
CD15
CD15 is a cluster of cell surface glycoproteins and glycolipids, also known as 3-fucosyl-N-acetyl-lactosamine or Lewis X. CD15 is a carbohydrate adhesion molecule that functions in cell-to-cell recognition processes. It is a distinguishing marker for human myeloid cells and mediates neutrophil adhesion to dendritic cells. Several studies have shown that CD15 expression is associated with prognosis and survival in a variety of cancers, such as breast cancer and Hodgkin’s lymphoma. (Download Biomarker Datasheet)
CD163
CD163 is a type I transmembrane protein belonging to the group B of the scavenger receptor cysteine-rich superfamily. It is involved in the clearance and endocytosis of haptoglobin-hemoglobin complexes and has been widely used to identify M2 type macrophage. The scavenging role of CD163 is critical to its anti-inflammatory response, and recent findings have shown the significance of CD163-positive macrophages in tumor progression. (Download Biomarker Datasheet)
CD20
CD20 is a B cell differentiation antigen expressed in B cell development from early pre-B cell stage to mature B cell stage but lost on differentiation into plasma cells. Its role is in regulating B- cell activation, proliferation and differentiation. Aberrant CD20 expression has been described in mainly B cell tumor types such as Burkitt lymphoma, non-Hodgkin lymphoma, and chronic lymphocytic leukemia (CLL). (Download Biomarker Datasheet)
CD206
CD206 is a C-type lectin that can be found on certain populations of macrophages and dendritic cells. Also known as mannose receptor C type 1 (MRC1), it is normally expressed on M2 macrophages. CD206 is thought to play a role in innate and adaptive immunity by acting as a pattern recognition receptor for various pathogens. (Biomarker Datasheet coming soon)
CD3
CD3 is a multimeric protein composed of 4 subunits (γ, δ, ε, ζ), which are part of the T-cell receptor (TCR). Engagement of CD3 induces downstream signaling events that result in T-cell activation. The specificity of the CD3 antigen for T cells and its appearance at all stages of T cell development makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms. (Download Biomarker Datasheet)
CD4
CD4 is a member of the immunoglobulin superfamily and is part of the TCR/CD3 complex, binding to MHC class II molecules and participating in signal transduction through recruitment of tyrosine kinase Lck. CD4 expression is used to identify helper T cells of which there are many different subsets including Th1, Th2, Th9, Th17, regulatory T cell, and follicular helper T cell. Each of these contributes to immune function through their unique cytokine profile. (Download Biomarker Datasheet)
CD45RO
CD45RO is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. Lack of CD45RO on T cells indicates naive T cell subsets while CD45RO expression indicated previous antigen exposure and defines the memory T cell subset. High density of CD45RO+ T cells in solid tumors is associated with a better prognosis. (Download Biomarker Datasheet)
CD56
CD56, also known as neural cell adhesion molecule (NCAM) is a transmembrane glycoprotein often considered a marker of neural lineage commitment due to its discovery site. CD56 expression is most strongly associated with natural killer (NK) cells but it has also been detected on other lymphoid cells, including gamma delta (γδ), T cells and activated CD8+ T cells, as well as dendritic cells. (Download Biomarker Datasheet)
CD68
CD68 is expressed on human macrophages and other mononuclear phagocytes. CD68 is a heavily glycosylated glycoprotein that is involved in ligand binding and is a member of the scavenger receptor family. CD68 functions in phagocytic activities and macrophage homing. An increased CD68+ macrophage index is associated with metastasis, shorter disease-free interval, poor prognosis, and reduced overall survival in multiple types of cancer. (Download Biomarker Datasheet)
CD8
CD8 is primarily expressed on cytotoxic T cells, but it can also be expressed on cortical thymocytes, dendritic cells and NK cells. CD8 is a transmembrane glycoprotein that is a co-receptor for the T cell receptor (TCR). CD8 binds MHC Class I to aid in antigen recognition and TCR-mediated activation. CD8 forms dimers of CD8ɑ and CD8β and clone C8/144B recognizes the alpha form of CD8. (Download Biomarker Datasheet)
CK
Cytokeratins play a cytoskeletal role in epithelial tissue and are an important component of intermediate filaments. These provide a structural framework for the cell and help resist mechanical stress. The mixture of AE1 and AE3 clones are able to detect a mixture of low and high molecular weight cytokeratins, thus identifying a broad range of cytokeratins. This marker can be used to identify the epithelial nature of tissue and tumors. (Download Biomarker Datasheet)
FoxP3
FoxP3, or Forkhead Box P3 is a transcription factor important in the development and inhibitory function of regulatory T cells (Tregs). FoxP3 functions by inhibiting cytokine production and T cell effector function, thus playing a crucial role in maintenance of immunological tolerance and control of immune responses against tumors and pathogens. (Download Biomarker Datasheet)
GrzB
Granzyme B (GrzB) is a serine protease stored in secretory granules of Cytotoxic T lymphocytes (CTLs) and Natural killer (NK) cells. Activated cytotoxic cells release granzyme B which enters the target cells where it can interact with cellular substrates to initiate cell death. (Download Biomarker Datasheet)
HLA-DR
Human leukocyte antigen (HLA) complex encodes the major histocompatibility complex (MHC). HLA-DR is the main isotype of 3 isotype (-DR, -DP, -DQ) responsible for presentation of antigens to T cells and B cells. Often, HLA-DR is used as a marker indicating the presence of antigen-presenting cells. HLA-DR expression in tumors has been shown to be positively associated with patient prognosis in some cancers such as colorectal cancer but is negatively associated with prognosis in other cancer types, such as glioma. (Download Biomarker Datasheet)
Ki67
Ki67 is a nuclear marker associated with cellular proliferation. Ki67 is present within the nucleus of cells undergoing division during interphase but is absent in quiescent cells. Ki67 can also be used as a prognostic indicator in certain cancers. (Download Biomarker Datasheet)
MHC II
Major Histocompability Complex II (MHC II) molecules are heterodimer complex that presents peptide antigen on the surface of the professional antigen presenting cells (APC’s) like macrophages and dendritic cells. Presentation of the antigen by MHC II complex is critical in CD4 activation and development of adaptive immune response. Along with APC’s, B cells and epithelial cells also present the MHC II molecule. (Biomarker Datasheet coming soon)
PD1
Programmed cell death protein 1, also known as PD1 and CD279, is an inhibitory receptor expressed by all T cells during activation. It regulates T cell effector functions during physiological responses, including acute and chronic infection, cancer, and autoimmunity. When PD-1 is bound to his ligand PD-L1, it prevents T cells from killing target cells including cancer cells. (Download Biomarker Datasheet)
PD-L1
Programmed cell death ligand 1 (PD-L1) is a type 1 transmembrane protein (B7-H1) that belongs to the B7 ligands family and may be expressed on both, hematopoietic cells (dendritic cells, macrophages, mast cells, T cells and B cells) and non-hematopoietic cells, including endothelial, epithelial and tumor cells. It plays an immunosuppressive role by inhibiting T-cell activity. Overexpression of PD-L1 by cancer cells may enable them to evade the host immune response, conferring a growth advantage to such tumors. (Download Biomarker Datasheet)
Sox10
Sox10 (Sry-related HMg-Box gene 10) is a nuclear transcription factor invovled in differentiation of neural crest progenator cells to melanocytes and maintanace of Schwann cells. Its high expression is observed in the melanocytic tumors of skin, soft tissue and primary as well as metastatic melanoma. (Download Biomarker Datasheet)
CK/Sox10
Cytokeratins play a cytoskeletal role in epithelial tissue and are an important component of intermediate filaments. These provide a structural framework for the cell and help resist mechanical stress. The mixture of AE1 and AE3clones can detect a mixture of low and high molecular weight cytokeratins, thus identifying a broad range of cytokeratins. This marker can be used to identify the epithelial nature of tissue and tumors. (Download Biomarker Datasheet)
SOX10 (Sry-related HMg-Box gene 10) is a nuclear transcription factor involved in differentiation of neural crest progenitor cells to melanocytes and maintenance of Schwann cells. Its high expression is observed in the melanocytic tumors of skin, soft tissue and primary as well as metastatic melanoma. (Download Biomarker Datasheet)
CD68/CD163
CD68 is expressed on human macrophages and other mononuclear phagocytes. CD68 is a heavily glycosylated glycoprotein that is involved in ligand binding and is a member of the scavenger receptor family. CD68 functions in phagocytic activities and macrophage homing. An increased CD68+ macrophage index is associated with metastasis, shorter disease-free interval, poor prognosis, and reduced overall survival in multiple types of cancer. (Download Datasheet)
CD163 is a type I transmembrane protein belonging to the group B of the scavenger receptor cysteine-rich superfamily. It is involved in the clearance and endocytosis of haptoglobin-hemoglobin complexes and has been widely used to identify M2 type macrophage. The scavenging role of CD163 is critical to its anti-inflammatory response, and recent findings have shown the significance of CD163-positive macrophages in tumor progression. (Download Biomarker Datasheet)
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Why Tissue Plex with InSituPlex®?
Ultivue provides researchers and scientists with multiplex biomarker assays and advanced image informatics for tissue phenotyping and digital pathology. Our proprietary InSituPlex® technology enables advanced exploration and interrogation of tissue samples for precision medicine research. These highly customizable solutions and scientific consultative approach strengthen and accelerate biomarker discovery and drug development programs.
InSituPlex® technology requires only a single antigen retrieval step before applying a mixture of DNA barcoded antibodies, which then bind to the desired array of antigens. After amplification, fluorescent probes are attached to those barcodes, allowing for visualization of the model using fluorescent microscopy. The InSituPlex® technology is available in up to 12-plex panel options.
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