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Presented at: AACR 2024

A novel method to minimize HIER-induced alterations on H&E staining in an integrated mIF-H&E workflow

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Relevant for

InSituPlex, mIF-H&E fusion

Description Discover a new workflow that enables you to effectively combine H&E staining with mIF InSituPlex® assays and avoid the heat-induced epitope retrieval (HIER) steps that are the root cause of altered H&E staining. We present data showing co-registration of H&E and mIF images with sub-micron accuracy, enabling highly accurate molecular characterization and cellular phenotyping.

Authors

Kevin Hwang, Grace Vezeau, Edyta Olejnik, Douglas Wood, Ruben Cardenes, Lauren Duro, Gourab Chatterjee, Je H. Lee

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