Why Tissue Plex with InSituPlex®?

Ultivue provides researchers and scientists with multiplex biomarker assays and advanced image informatics for tissue phenotyping and digital pathology. Our proprietary InSituPlex® technology enables advanced exploration and interrogation of tissue samples for precision medicine research. These highly customizable solutions and scientific consultative approach strengthen and accelerate biomarker discovery and drug development programs.

InSituPlex® technology requires only a single antigen retrieval step before applying a mixture of DNA barcoded antibodies, which then bind to the desired array of antigens. After amplification, fluorescent probes are attached to those barcodes, allowing for visualization of the model using fluorescent microscopy. The InSituPlex technology is available with panel options of up to 12 targets.

InSituPlex® Technology

Fast and Simple Workflow

Slide Preparation

The first step involves slide preparation (dewaxing) and single antigen retrieval from FFPE samples. This simplified process requires only one retrieval step, preserving the tissue.

Antibody Binding

The antibody barcode conjugates are incubated on the slide to stain the biomarkers. Each antibody is labelled with a DNA barcode that uniquely identifies it for detection. Up to 12 different antibodies can be added simultaneously. Only one single staining step is required, eliminating the need for secondary antibodies.

Barcode Amplification

During the next step the barcodes linked to the antibodies are amplified simultaneously and at the same rate. The extended barcodes do not interfere with the immediate surroundings so there is no steric hindrance or epitope masking.

Detection

The complementary fluorescent DNA probes are added and hybridize to the barcode targets. The samples are then ready for imaging.

Signal Removal

For additional rounds, signal must be removed by using exchange technology. This involves the gentle removal of fluorescent DNA probes, while keeping barcodes and tissue integrity intact. The slide is now ready for additional fluorescent DNA probes.