Assay Development
Transparency & constant communication are the principles that guide our assay development team and ensure your needs are met. Ultivue offers multiplex IF services through its proprietary InSituPlex® DNA-barcoding and antibody staining technology.
Explore our lab
Click on each station in the lab below to learn more about our assay development services.
Explore our lab
Click on each station in the lab below to learn more about our assay development services.
Assay Development
Our in-house team specializes in assay development, resulting in the rapid turnaround of custom assays and panels for any species. The development of non Ultivue menu markerscan come from commercially sourced, or customer provided reagents. With each project, image reviews and optimized assay conditions are provided to our customers.
Tissue preparation
At the start of any project, tissue undergo FFPE slide sectioning, for preparation ahead of staining. Our lab is overseen by trained histopathologists and histology scientists that can bring the necessary attention to detail required to support, develop, validate and implement your assay through the drug development pipeline.
Staining
Ultivue’s InSituPlex® mIF staining allows multiplexed staining of up to 12 biomarkers with H&E – on the same sample-using the Leica Bond Rx.
Our signal amplification technology creates a linear relationship of biomarker abundance and staining intensity with superior signal to noise ratio, ideal for image analysis.
Scanning & Imaging
Whole slide scanning & imaging are performed using the Zeiss Axioscan by trained technicians. As a CLIA-certified lab, we ensure regulatory compliance and data integrity for your projects. Rest assured that you will receive accurate, reliable, and timely results.
Analysis
Ultivue can provide complete phenotype analysis, advanced analysis and data mining based on your scientific question. With a complete representation of the tumor landscape including compartments, cell phenotypes and interactions, you areready for scientific exploration.
Final Report: Custom Data Delivery
At the end of each milestone, you will receive a report on the projects progress. Upon project completion, images, data results and final report are delivered by one of our data
sharing solutions.
Assay Development
Our in-house team specializes in assay development, resulting in the rapid turnaround of custom assays and panels for any species. The development of non Ultivue menu markerscan come from commercially sourced, or customer provided reagents. With each project, image reviews and optimized assay conditions are provided to our customers.
Tissue preparation
At the start of any project, tissue undergo FFPE slide sectioning, for preparation ahead of staining. Our lab is overseen by trained histopathologists and histology scientists that can bring the necessary attention to detail required to support, develop, validate and implement your assay through the drug development pipeline.
Staining
Ultivue’s InSituPlex® mIF staining allows multiplexed staining of up to 12 biomarkers with H&E – on the same sample-using the Leica Bond Rx.
Our signal amplification technology creates a linear relationship of biomarker abundance and staining intensity with superior signal to noise ratio, ideal for image analysis.
Scanning & Imaging
Whole slide scanning & imaging are performed using the Zeiss Axioscan by trained technicians. As a CLIA-certified lab, we ensure regulatory compliance and data integrity for your projects. Rest assured that you will receive accurate, reliable, and timely results.
Analysis
Ultivue can provide complete phenotype analysis, advanced analysis and data mining based on your scientific question. With a complete representation of the tumor landscape including compartments, cell phenotypes and interactions, you areready for scientific exploration.
Final Report: Custom Data Delivery
At the end of each milestone, you will receive a report on the projects progress. Upon project completion, images, data results and final report are delivered by one of our data
sharing solutions.
Talk to our experts
Our pathologists, histopathology experts, and image analysis and AI scientists ensure that your scientific research needs are met. The same experts are involved throughout the entire length of a project ensuring consistency from project inception all the way to delivery of the results.
Contact usAssay Development Milestones
Spatial phenomics expands the depth of information possible from a single tissue section
InSituPlex® Technology
Why Tissue Plex with InSituPlex®?
Ultivue provides researchers and scientists with multiplex biomarker assays and advanced image informatics for tissue phenotyping and digital pathology. Our proprietary InSituPlex® technology enables advanced exploration and interrogation of tissue samples for precision medicine research. These highly customizable solutions and scientific consultative approach strengthen and accelerate biomarker discovery and drug development programs.
InSituPlex® technology requires only a single antigen retrieval step before applying a mixture of DNA barcoded antibodies, which then bind to the desired array of antigens. After amplification, fluorescent probes are attached to those barcodes, allowing for visualisation of the model using fluorescent microscopy. The InSituPlex technology is available in 4, 8, 12-plex panel options.
Ultivue Staining Technology
Not sure where to start?
We can help you to scope your project and discuss timelines and deliverables.
Assay Development | Image Analysis | Data Mining
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