Immuno8 FixVUE™ Panel

Characterize T-cell subsets, immunosuppressive cells and interactions along the PD-L1/PD-1 checkpoint axis with this comprehensive immuno-oncology focused panel. The Immuno8 FixVUE™ panel enables phenotyping of at least 14 distinct cell types in the tumor microenvironment. Use this panel to dive deeper into the biology or find the key differences between samples and scale down to the essential markers to move your science forward.

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This antibody panel consists of the following markers:

CD3
CD4
CD8
CD68
FoxP3
PD-1
PD-L1
CK/SOX10

Cell Phenotypes

Cell Phenotyping with the Immuno8 FixVUE™ Panel

The Immuno8 FixVUE™ antibody panel enables users to identify and characterize T-cell subsets, immunosuppressive cells, and interactions along the PD-L1/PD-1 checkpoint axis. CD3 is a marker of T cells. CD4 is expressed on the surface of T helper cells. CD8 is a marker for cytotoxic immune cells (primarily cytotoxic T cells). CD68 is a marker of monocytes and macrophages. FoxP3 is a transcription factor and marker of cellular activation.

The co-expression of CD3, CD4, and FoxP3 indicates the regulatory T cell (T-reg) phenotype. PD-1 marks exhausted or suppressive T cells (CD3+/PD-1+) and can serve a pro-tumor function to help tumors evade the immune system. PD-L1 is an immune checkpoint marker that can be expressed on both macrophages (CD68+/PD-L1+) and tumor cells (CK/SOX10+/PD-L1). SOX10 is a marker of melanoma tumor cells while pan-cytokeratin detects carcinoma tumor cells.

Markers

Phenotype

CD3 CD4 CD8 CD68 FoxP3 PD-1 PD-L1 CK/SOX10

T cells

T helper cells

Cytotoxic T cell

FoxP3+ T cells

Exhausted T cells

Regulatory T cells (T-reg)

CD4/CD8 Double-positive T cells

CD8+ Regulatory T cell (CD8 T-reg)

Exhausted, Cytotoxic T cell

Macrophage

Immunosuppressive macrophage

Tumor cell

Immune-evading tumor cell

This multiplexed immunofluorescence (mIF) panel allows for the spatial identification of single biomarkers and co-expression of multiple markers in cells enabling the observation of several biologically relevant phenotypes. The above is a partial of the 256 distinct binary phenotypes that this panel can identify. The number of phenotypes increases if binned marker intensities are taken into account (e.g.PD-1, PD-L1, FoxP3 expression levels)

Phenotype

Markers

Markers

Phenotype, Markers

T cells

CD3

T helper cells

CD3 CD4

Cytotoxic T cell

CD3 CD8

FoxP3+ T cells

CD3 FoxP3

Exhausted T cells

CD3 PD-1

Regulatory T cells (T-reg)

CD3 CD4 FoxP3

CD4/CD8 Double-positive T cells

CD3 CD4 CD8

CD8+ Regulatory T cell (CD8 T-reg)

CD3 CD8 FoxP3

Exhausted, Cytotoxic T cell

CD3 CD8 PD-1

Macrophage

CD68

Immunosuppressive macrophage

CD68 PD-L1

Tumor cell

CK/SOX10

Immune-evading tumor cell

PD-L1 CK/SOX10

Product Biology

Markers

Main Cell Type

Markers,Main Cell Type

CD3

T cells

CD3, T cells

Identifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.

CD4

Helper T cell

CD4, Helper T cell

CD4 binds to MHC class II molecules and participates in signal transduction processes. Its expression is used to identify helper T cells of which there are many different subsets each contributing to the overall immune function through their unique cytokine profile.

CD8

Cytotoxic T cells

CD8, Cytotoxic T cells

Cytotoxic T cells are responsible for mediating apoptosis of cancer cells through a variety of mechanisms that include the release of granzyme B from the T cells.

CD68

Marophages

CD68, Marophages

CD68 is expressed on human macrophages and other mononuclear phagocytes. CD68 functions in phagocytic activities and macrophage homing. An increased CD68+ macrophage index is associated with metastasis, poor prognosis, and reduced overall survival in multiple types of cancer.

FoxP3

Regulatory T cell

FoxP3, Regulatory T cell

FoxP3 is a transcription factor important in the development and inhibitory function of regulatory T cells (Tregs). FoxP3 functions by inhibiting cytokine production and T cell effector function, thus playing a crucial role in maintenance of immunological tolerance and control of immune responses against tumors and pathogens.

PD-1

Exhausted T cell

PD-1, Exhausted T cell

Programmed death receptor-1 (PD-1), an immune checkpoint molecule, is over expressed in cancer, leading to increased T cell exhaustion and a decreased anti-tumor response.

PD-L1

T cells, Monocytes, NK cells and Macrophages

PD-L1, T cells, Monocytes, NK cells and Macrophages

PD-L1 is expressed on the surface of tumor cells and it is able to bind to PD-1 on the surface of activated T cells and B cells. The binding of PD-L1 to PD-1 leads to an immunosuppressive effect and allows the tumor to evade immune destruction.

CK/SOX10

Tumor cells

CK/SOX10, Tumor cells

Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.