Description Innovative and efficient translational research tools enabling a better understanding of the tumor and its microenvironment are critical for the development of digital pathology. Current immunohistochemistry (IHC) methods limit the depth of information from a single tissue sample to a single target in the case of chromogenic staining, or to sample morphology and general cell identification in the case of hematoxylin and eosin (H&E). Accurate phenotyping of each cell must be performed with a single section, as serial sections may not contain the same cells, especially small immune cells such as T cells. Multiplex immunofluorescence (mIF) methods have been established to provide insights into a wide number of markers of interest and their spatial context in a single sample. Here, we demonstrate a new research approach combining multiplexed detection of protein markers with standard H&E pathology review in tumor samples, in a streamlined, single-day sample-to-answer workflow