T-act FixVUE™ Panel
Determine whether T cells have the potential to mediate cell death. This T cell activation 4-plex/5-color panel enables co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death.
View additional information (PDF)This antibody panel consists of the following markers:
Cell Phenotypes
Cell Phenotyping with the T-act FixVUE Panel��
The T-act FixVUE antibody panel enables users to identify activated T cells. CD3 is a marker of T cells. Granzyme B is produced, stored, and released by cytotoxic T cells to mediate cellular death in combination with perforin. Ki67 is a nuclear protein that is a marker of proliferation. Co-expression of CD3, Granzyme B, and Ki67 indicates proliferating cytotoxic T cells. SOX10 is a tumor marker for melanomas while PanCK detects carcinomas (provided in a cocktail).
Markers
Phenotypes |
CD3 | Granzyme B | Ki67 | PanCK/SOX10 |
T cells |
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Cytotoxic cells |
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Proliferating cells |
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Carcinoma or Melanoma |
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Cytotoxic T cells |
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Proliferating T cells |
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Proliferating cytotoxic cells |
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Proliferating cytotoxic T cells |
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Proliferating tumour cells |
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Phenotypes
Markers
Phenotypes, Markers
T cells
Cytotoxic cells
Proliferating cells
Carcinoma or Melanoma
Cytotoxic T cells
Proliferating T cells
Proliferating cytotoxic cells
Proliferating cytotoxic T cells
Proliferating tumour cells
Product Biology
Markers
Main Cell Type
Markers,Main Cell Type
CD3
T cells
CD3, T cells
Identifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.
Granzyme B
B Cytotoxic T cells and NK cells
Granzyme B, B Cytotoxic T cells and NK cells
Granzyme B is produced, stored, and released by cytotoxic cells to mediate cellular death in combination with Perforin.
Ki67
Proliferating cells
Ki67, Proliferating cells
Ki67 is expressed within the nucleus during the phases of mitosis and is strongly associated with cell proliferation.
CK/Sox10
Tumor cells
CK/Sox10, Tumor cells
The panel may be used to interrogate many tumor sample types for Research. A cocktail of optimized reagents for the detection of pan-Cytokeratin and Sox10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.
