U-VUE®

When your assay requirements are unique, start with the extensive menu of pre-optimized U-VUE markers to build your panel. Fully customizable, and our experts on hand to help guide your assay development, create a robust assay panel that will enable powerful spatial insights into the tissue microenvironment.

Develop customized biomarker panels to get the spatial insights that matter to you.


U-VUE biomarker

U-VUE biomarker

Checkpoints

Tumor cells use various tricks to escape the immune system, such as activating immune checkpoint pathways. Today, hundreds of clinical trials are incorporating immune checkpoint inhibitor (ICI) therapeutics.

  • CD27
  • CD28
  • CD40
  • CD70
  • CD73
  • CD80
  • CD96
  • CD155
  • CTLA4
  • IDO1
  • LAG3
  • PD-1
  • PD-L1
  • TIGIT
U-VUE biomarker

Exhaustion

T cell exhaustion arises from persistent T cell activation, which may explain why surface markers of exhausted T cells are inter-twined with activated T cells. Ex-hausted T cells start producing much lower amounts of immune response-stimulating proteins, and generally become less able to kill tumor cells.

  • CD8
  • CD39
  • CD68
  • CTLA4
  • LAG3
  • PD1
  • TIGT
U-VUE biomarker

TAMs

Tumor-associated macrophages represent a major component of myeloid cells in tumors. TAMs polarize to either M1, anti-tumor activity, or M2, which leads to tumor promotion. The balance between these two polarized macrophages is important for cancer therapies and correlates with clinical outcome.

  • Arg1
  • CD11c
  • CD163
  • CD206
  • CD68
  • CD86
  • HLA-DR
  • INOS
U-VUE biomarker

Myeloid

Myeloid cells are potential predictive biomarkers in checkpoint blockade-treated patients. Myeloid-derived suppressor cells and monocytes, the expansion of neutrophils, eosinophils and immature myeloid cells reflect the immunosuppressive status of the TME during immunotherapy.

  • Arg1
  • CD11b
  • CD14
  • CD15
  • CD16
  • CD68
  • CD163
  • HLA-DR
U-VUE biomarker

Functional

These include markers that can inform the cellular functional state (e.g., Ki-67 for proliferation and granzyme B for cytotoxicity)

  • CK
  • Desmin
  • GrzB
  • Ki67
  • Sox10
  • TCF1/TCF7
U-VUE biomarker

Dendritic

DCs are mediators of the innate and adaptive immune responses. They upregulate MHC molecules and costimulatory receptors, present antigens to naive T cells, and produce polarizing cytokines that effect T cell differentiation and activation.

  • CD3
  • CD4
  • CD11c
  • CD20
  • CD56
  • CD68
  • CD138
  • Clec9a
  • Clec10a
  • MHC II
U-VUE biomarker

Stromal

The TME or stromal is a multicellular system composed of cells from mesenchymal,
endothelial, and hematopoietic origins arranged in the extracellular matrix (ECM). Secreted products from a variety of cells and components drive the intratumoral environment.

  • Alpha-SMA
  • CD31
  • CD299
U-VUE biomarker

TLS

Tertiary lymphoid structures are ectopic aggregates of lymphocytes and antigen-presenting cells that develop in non-lymphoid tissues at sites of chronic inflammation. There is a close association between tumor-associated TLSs and favorable clinical outcomes in most types of cancers.

  • CD3
  • CD4
  • CD20
  • CD56
  • CD68
  • CD138
U-VUE biomarker

Cell Signaling

Therapeutic modalities inhibit tumor growth and metastasis by blocking commonly overexpressed signal transduction molecules. They comprise signalling pathways that cause profound effects on cells; apoptosis, proliferation and migration but are often aberrantly expressed in cancer cells.

  • Bcl-2
  • Bcl-cl
  • Beta-Catenin
  • Bim
  • Ki67
  • MCL-1
U-VUE biomarker

T-Cell Regulation

The crucial role of T cell lymphocytes is as the main regulators and effectors in anti-tumor immunity. Many studies have revealed their role in immune surveillance and the vital contribution made by adaptive immune cells in suppressing the formation of tumors.

  • CD3
  • CD4
  • CD8
  • CD27
  • CD38
  • CD45RO
  • CD70
  • FoxP3
  • GrzB
  • LAG3
  • PD1
  • PD-L1
U-VUE biomarker

TILS

Tumor infiltrating lymphocytes consist of any lymphocytic cell populations that have invaded the tumor. TILs are described in several tumor types and immune checkpoint inhibitor therapy in cancer patients has been linked to the quality and magnitude of T cell, NK cell, and B cell responses.

  • CD3
  • CD4
  • CD8
  • CD20
  • CD38
  • CD45RO
  • CD56
  • CD68
  • CD335
U-VUE biomarker

B cells

B cells and plasma cells located in tumors can have important roles in shaping anti-tumor immune responses. B cells can present cognate tumor-derived antigens to T cells and the densities of tumor-associated plasma cells may predict improved cancer prognosis.

  • BCMA
  • CD19
  • CD20
  • CD22
  • CD27
  • CD38
  • CD138
  • Pax5
  • MHC II
All U-VUE biomarkers

All U-VUE biomarkers

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antibody selection and biomarker strategy: ensure that your design a panel optimized for the best data

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assay development: get assistance to ensure that your assay will translate from research to clinical use

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pathological assessment: our pathologists can assist with tailoring your project to maximize its relevance

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image and data analysis: computational expertise to find the key features and develop robust algorithms

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With our extensive U-VUE marker menu we can help you build the ideal panel for your research.

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