InSituPlex
5846 Utilizing image analysis to characterize an 8-plex immunofluorescence immune checkpoint biomarker panel within the tumor tissue microenvironment
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Presented at: AACR 2020

Utilizing image analysis to characterize an 8-plex immunofluorescence immune checkpoint biomarker panel within the tumor tissue microenvironment

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Collaboration, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

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Want to meet with us? Check out our upcoming events.

Categories: 5874 Introduction of a robust workflow for the whole-slide acquisition and co-registration of multiplex immunofluorescence tissue images for analysis of a 9-color, 8-marker immunophenotyping assay
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Introduction of a robust workflow for the whole-slide acquisition and co-registration of multiplex immunofluorescence tissue images for analysis of a 9-color, 8-marker immunophenotyping assay

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Relevant for

Collaboration, FixVUE, InSituPlex

Description Profiling multiple cell types in tissue samples requires more markers than can be supported by conventional approaches to immunohistochemistry. InSituPlex® multipleximmunofluorescence (mIF) technology enables biomarker multiplexing int issue samples with an automated workflow to support discovery of immunecell signatures in the tumor immunemicroenvironment (TiME).Here we have designed and tested an 8- plex immuno phenotyping panel using the InSituPlex® approach to detect and classify T cells, macrophages, and tumor cell in non- small cell lung cancer (NSCLC) and colorectal cancer (CRC) FFPE tissue.

Authors

Anne Hellebust, Kyla Teplitz, Katir K Patel, Karan Sharma, Amanda Bares, Mael Manesse, Mark Burton, Bonnie Phillips, Sean Downing and Kate Lillard

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5871 High-multiplex automated staining and scanning workflow with Ultivue InSituPlex technology and the Olympus SLIDEVIEW VS200 slide scanner
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High-multiplex automated staining and scanning workflow with Ultivue InSituPlex technology and the Olympus SLIDEVIEW VS200 slide scanner

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Relevant for

Collaboration, FixVUE, InSituPlex

Description Overview highlighting InSituPlex technology coupled to the SLIDEVIEW VS200 scanner to produce high-quality, aligned images of eight targets in FFPE tumor sections

Authors

Amanda Bares, Alec De Grand, Douglas Wood and Maël Manesse

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5853 Same-slide multiplex immunofluorescence and H&E staining
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Presented at: AACR 2020

Same-slide multiplex immunofluorescence and H&E staining

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Relevant for

FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Autors

Douglas Wood, Heike Boisvert, Sean R. Downing and Maël Manesse

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5863 Looking beyond the assay: Comparison of multiplex chromogenic and fluorescent immuno-histochemistry for standardized immune oncology profiling in non-small cell lung carcinoma patients
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Presented at: AACR 2020

Looking beyond the assay: Comparison of multiplex chromogenic and fluorescent immuno-histochemistry for standardized immune oncology profiling in non-small cell lung carcinoma patients

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Relevant for

Collaboration, FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5857 Tissue-based characterization of T cell exhaustion in inflammatory bowel disease and colorectal cancer using multiplex IHC
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Presented at: AACR 2020

Tissue-based characterization of T cell exhaustion in inflammatory bowel disease and colorectal cancer using multiplex IHC

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Relevant for

FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5845 Tumour immunity signatures to expand current diagnostic approaches in mismatch repair deficient cancers in the context of Lynch syndrome through InSituPlex technology and Tissue Phenomics integration
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Presented at: AACR 2020

Tumour immunity signatures to expand current diagnostic approaches in mismatch repair deficient cancers in the context of Lynch syndrome through InSituPlex technology and Tissue Phenomics integration

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Relevant for

FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5821 A phase I clinical trial on intratumoral administration of autologous CD1c (BDCA-1)+ myeloid dendritic cells (myDC) plus talimogene laherparepvec (T-VEC) in patients with advanced melanoma
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Presented at: AACR 2020

A phase I clinical trial on intratumoral administration of autologous CD1c (BDCA-1)+ myeloid dendritic cells (myDC) plus talimogene laherparepvec (T-VEC) in patients with advanced melanoma

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Relevant for

FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Autors

Douglas Wood, Heike Boisvert, Sean R. Downing and Maël Manesse

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5827 Use of Ultivue InSituPlex®Multiplex Immunofluorescence to Localize and Quantify Regulatory T Lymphocytes in Crohn’s Disease and Ulcerative Colitis
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Presented at: AACR 2020

Use of Ultivue InSituPlex®Multiplex Immunofluorescence to Localize and Quantify Regulatory T Lymphocytes in Crohn’s Disease and Ulcerative Colitis

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Relevant for

Collaboration, FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5973 A multiplex immunofluorescence assay to assess immune checkpoint inhibitor targeted CD8 activation and tumor co-localization in FFPE tissues Copy
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Presented at: AACR 2020

A multiplex immunofluorescence assay to assess immune checkpoint inhibitor targeted CD8 activation and tumor co-localization in FFPE tissues Copy

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Relevant for

Collaboration, FixVUE, InSituPlex

Authors

Alison Bigley, Lorcan Sherry, Nicole Stille, Douglas Wood and Sean Downing

Autors

Douglas Wood, Heike Boisvert, Sean R. Downing and Maël Manesse

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5882 Case Study: A multiplex immunofluorescence assay to assess immune checkpoint inhibitor-targeted CD8 activation and tumor co-localization in FFPE tissues
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Case Study: A multiplex immunofluorescence assay to assess immune checkpoint inhibitor-targeted CD8 activation and tumor co-localization in FFPE tissues

Register here for download

Relevant for

Collaboration, FixVUE, InSituPlex

Description Profiling multiple cell types in tissue samples requires more markers than can be supported by conventional approaches to immunohistochemistry. InSituPlex® multipleximmunofluorescence (mIF) technology enables biomarker multiplexing int issue samples with an automated workflow to support discovery of immunecell signatures in the tumor immunemicroenvironment (TiME).Here we have designed and tested an 8- plex immuno phenotyping panel using the InSituPlex® approach to detect and classify T cells, macrophages, and tumor cell in non- small cell lung cancer (NSCLC) and colorectal cancer (CRC) FFPE tissue.

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

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