Senior Scientists, Product Development
Relevant for
FlexVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Yvette is a board-certified Histotechnologist with nearly 2 decades of experience in Anatomic Pathology. She has served as a technical lead at clinical academic and contract research pathology laboratories. Yvette has extensive expertise in histologic technique assay development and multispectral digital pathology.
Presented at: SITC 2021
Relevant for
Collaboration, FixVUE, Imaging mass cytometry, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Andrew A. Quong, Jordan Nieto, Derek Quong, Amanda Esch, Mael Manesse, Gourab Chatterjee, Devan Fleury, Keith A. Wharton Jr,Kirsteen H. Maclean, Mark Rees, Jeppe Thagaard, Fabian Schneider, Dan Winkowski and James Mansfield.
Relevant for
FixVUE, InSituPlex
Description Discover more about Multiplexed Immunofluorescence. Our new infographic discusses some of the differences of traditional IHC technology with our unique ISP technology enabling a comprehensive study of cellular composition, cellular functions, and cell-cell interaction, within the spatial context of the tumor.
Presented at: SITC 2021
Relevant for
FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Andrew A. Quong, Jordan Nieto, Derek Quong, Amanda Esch, Mael Manesse, Gourab Chatterjee, Devan Fleury, Keith A. Wharton Jr,Kirsteen H. Maclean, Mark Rees, Jeppe Thagaard, Fabian Schneider, Dan Winkowski and James Mansfield.
Presented at: SITC 2021
Relevant for
Collaboration, FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Relevant for
FixVUE, InSituPlex, U-VUE
Description Discover more about Multiplexed Immunofluorescence. Our new infographic discusses some of the differences of traditional IHC technology with our unique ISP technology enabling a comprehensive study of cellular composition, cellular functions, and cell-cell interaction, within the spatial context of the tumor.
Senior Field Applications Scientist, Ultivue
Relevant for
FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Yvette is a board-certified Histotechnologist with nearly 2 decades of experience in Anatomic Pathology. She has served as a technical lead at clinical academic and contract research pathology laboratories. Yvette has extensive expertise in histologic technique assay development and multispectral digital pathology.
Associate Director, Scientific Affairs; Senior Scientist, Visiopharm
Relevant for
Collaboration, FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Yvette is a board-certified Histotechnologist with nearly 2 decades of experience in Anatomic Pathology. She has served as a technical lead at clinical academic and contract research pathology laboratories. Yvette has extensive expertise in histologic technique assay development and multispectral digital pathology.
Dr. Fabian Schneider is part of Visiopharm’s R&D and Product Management team, responsible for phenotyping products as well as service projects for custom APP development. Fabian has over 10 years of international experience in cancer biology and immuno-oncology, working in academic research labs, clinical research teams and computational pathology groups in both academia and biopharma. Fabian received his Dr phil. nat. in Cell Biology in 2011 from the Johan Wolfgang Goethe University Frankfurt, Germany.
Presented at: AACR 2021
Relevant for
Collaboration, FixVUE, InSituPlex
Description Understanding the complexities of the tumor micro-environment in detail can vastly improve the accuracy of immuno-oncology research and accelerate the discovery of potential immunotherapy targets. Multiplexed immunofluorescence (mIF) assays have emerged as a critical approach for identifying complex cellular phenotypes in the tumor micro-environment, powered by the value of the spatial correlation of such phenotypes in a tissue specimen. Although many methods for multiplexed IF exist, they are often costly and require time-consuming assay development and long imaging times. The Ultivue UltiMapper kits enable rapid, pre-optimized staining of multiple targets simultaneously in a single FFPE tissue section. This technology is ready-to-use with conventional automated staining workflows and commercially available automated imaging systems. Here, we demonstrate the potential of accurate cellular phenotyping using the InSituPlex technology with fast whole-slide scanning using ZEISS Axioscan.Z1 an automated slide scanning system for fluorescence and transmitted light applications with modern cameras, a sophisticated focusing method and a powerful imaging software. The compounded effect of integrating these technologies can significantly improve the throughput of immuno-oncology research. A 4-target immunostaining assay was performed on multiple FFPE tumor sections using UltiMapper reagents. Briefly, slides were stained with a cocktail of primary antibodies using a Leica Biosystems BOND RX autostainer. Post-staining, the slides were coverslipped and imaged on ZEISS Axioscan.Z1. The resulting images were analyzed using IndicaLabs HALO analysis software. In this poster, we report on an efficient and streamlined workflow for mIF staining, imaging, and analysis of tumor samples. After nuclear segmentation of the resulting images, T-cell and macrophage populations were subtyped and identified through spatial identification of relevant biomarkers. The combined workflow presented here allows a user to get 4-plex, whole slide immunofluorescence images of FFPE tumor tissues within 6 hours. The automated whole slide scanning capability of ZEISS Axioscan.Z1 also allows a user to image up to 100 slides with minimal external input. The combination of optimized, fast staining UltiMapper reagents with ZEISS Axioscan.Z1 automated whole-slide fluorescence scanner enables rapid, deep immunoprofiling within the spatial context of the tumor, empowering translational and immuno-oncology research.
Catherine Benson, Keith Wharton, Gourab Chatterjee, Markus Neumann, Volker Döring and Maël Manesse
Presented at: SITC 2021
Relevant for
Collaboration, FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Andrew A. Quong, Jordan Nieto, Derek Quong, Amanda Esch, Mael Manesse, Gourab Chatterjee, Devan Fleury, Keith A. Wharton Jr,Kirsteen H. Maclean, Mark Rees, Jeppe Thagaard, Fabian Schneider, Dan Winkowski and James Mansfield.
Relevant for
FixVUE, FlexVUE, InSituPlex, U-VUE
Description Discover more about Multiplexed Immunofluorescence. Our new infographic discusses some of the differences of traditional IHC technology with our unique ISP technology enabling a comprehensive study of cellular composition, cellular functions, and cell-cell interaction, within the spatial context of the tumor.
Associate Director, Scientific Affairs Ultivue ;Tissue Hybridization Scientific Lead, Queens University Belfast
Relevant for
FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Yvette is a board-certified Histotechnologist with nearly 2 decades of experience in Anatomic Pathology. She has served as a technical lead at clinical academic and contract research pathology laboratories. Yvette has extensive expertise in histologic technique assay development and multispectral digital pathology.
At Queens University Belfast, Matt is the Scientific Lead for Tissue Hybridization and Digital Pathology in the Precision Medicine Centre of Excellence. Matt is responsible for the TH&DP team. Their remit was to deliver academic research studies, clinical trials, and industry projects within the PMC.
Presented at: AACR 2020
Relevant for
Collaboration, FixVUE, InSituPlex
Presented at: SITC 2021
Relevant for
Collaboration, FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
Presented at: SITC 2021
Relevant for
Collaboration, FixVUE, InSituPlex
Description Get an overview of our new addition to the Ultivue products, FlexVUE. Find out how the new flexible 8-plex panel can be tailored to allow investigation of specific cell types in the tumor microenvironment without added development time or costs.
