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8726 Precise and Robust Immune Profiling: Validation of a Flexible Multiplex Immunofluorescence Workflow
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webinar
Yvette Cajigas & Angela Vasaturo, PhD

Director of Product Development at Ultivue, Inc. & Director of Scientific Affairs, Ultivue

Precise and Robust Immune Profiling: Validation of a Flexible Multiplex Immunofluorescence Workflow

1 hour

Relevant for

InSituPlex

Description Though widely recognized that mIF assays have critical implications for clinical translation, validation of mIF staining that allows for flexibility in biomarker selection is a barrier for mIF integration into clinical practice. Join Yvette Cajigas and Angela Vasaturo, experts in assay development and biomarker strategy, and learn about a simple approach to evaluate sensitivity and reproducibility – without sacrificing the flexibility needed to answer biological questions required for evaluating specific treatment options. Take a deep dive into the world of multiplex immunofluorescence and see first-hand how Ultivue’s OmniVUE panels use its patented InSituPlex® technology to advance precision therapies in clinical trials.

Key Takeaways

Understand the challenge of mIF validation and its critical implications for clinical translation

Discover a simple method to assess sensitivity and reproducibility using the InSituPlex® (ISP) staining workflow without sacrificing flexibility

See how single markers in the multiplex ISP assay demonstrate high reproducibility and steric independence, making it a reliable approach.

Meet the presenters

Yvette Cajigas:

Yvette Cajigas is Director of Product Development at Ultivue, and is a board-certified histotechnologist with nearly 2 decades of experience in Anatomic Pathology. She has served as a technical lead at clinical academic and contract research pathology laboratories, and has extensive expertise in histologic technique assay development and multispectral digital pathology.

Angela Vasaturo, PhD

Dr. Angela Vasaturo is Director of Scientific Affairs at Ultivue. Prior to Ultivue, Angela was a Senior Researcher in Dr. Jerome Galon’s Laboratory of Integrative Cancer Immunology at the Cordeliers Research Center. Angela was among the first in Europe to be involved in the development of multiplex IHC and multispectral imaging and analysis of up to six immunofluorescence markers, and today is considered a leading European expert in multiplex IHC techniques, digital pathology, spatial biology, and tissue imaging.

 

Ultivue

Author
Yvette Cajigas

Director of Product Development at Ultivue, Inc.

Author
Angela Vasaturo, PhD

Director of Scientific Affairs at Ultivue, Inc.

Categories: 8019 Unraveling the Tumor Microenvironment Using Custom mIF Panels & Spatial Tissue Analysis
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Angela Vasaturo, PhD

Director of Scientific Affairs, Ultivue

Unraveling the Tumor Microenvironment Using Custom mIF Panels & Spatial Tissue Analysis

1 hour

Relevant for

InSituPlex

Description A persistent challenge in the field of immunotherapy is the fact that only a minority of patients respond to treatment. This is especially true for therapies which rely on immune activation, such as checkpoint inhibitors, due to the complex and heterogeneous immune escape mechanisms which can develop in each patient. Therefore, the development of robust biomarkers, which enable rational patient selection and the design of precise combination therapies, is key for the continued success and improvement of cancer treatments. Multiplex immunofluorescence (mIF) has emerged to complement the conventional IHC by providing highly reproducible, efficient, quantitative, and standardized assays for the analysis of formalin-fixed paraffin-embedded (FFPE) tissue samples. The simultaneous antibody-based detection of multiple markers on a single tissue section can address the low tissue availability from precious and rare donors, establishing mIF as an important assay in the study of the tumor microenvironment and one which can help to maximize the opportunity for patients to benefit from personalized immunotherapies. To enable the transition of mIF assays into clinical applications, a flexible, easy- to- use, and reproducible assay is needed to identify predictive biomarkers. Multiplex immunofluorescence gives researchers the opportunity to study the spatial relationships in the tumor microenvironment, providing access to a wealth of data and information about its immune contexture. The purpose of this presentation is to convey to scientists and pathologists the reasons why reproducible assays are essential for biomarker discovery during clinical trials, how multiplex mIF assays are a great tool for immune profiling the tumor microenvironment, and how spatial tissue analysis can help to make data-driven decisions.

Key Takeaways

Learn why reproducible assays are essential for biomarker discovery

Discover how flexible mIF assays meet the needs for tumor microenvironment research within clinical trials

Gain an understanding of how spatial tissue analysis can help you make data-driven decisions

Meet the presenter

Dr. Angela Vasaturo is Director of Scientific Affairs at Ultivue. Prior to Ultivue, Angela was a Senior Researcher in Dr. Jerome Galon’s Laboratory of Integrative Cancer Immunology at the Cordeliers Research Center. Angela was among the first in Europe to be involved in the development of multiplex IHC and multispectral imaging and analysis of up to six immunofluorescence markers, and today is considered a leading European expert in multiplex IHC techniques, digital pathology, spatial biology, and tissue imaging.

Author
Angela Vasaturo, PhD
Director of Scientific Affairs, Ultivue
Categories: 6709 Spatially defining the future of cancer biology with advanced multiplexed immunofluorescence
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Troy Bloom

Senior Field Application Scientist, Ultivue

Spatially defining the future of cancer biology with advanced multiplexed immunofluorescence

30 minutes

Relevant for

InSituPlex

Description Immunotherapy has transformed the treatment of metastatic and recurrent solid tumors. Advances in technology in the past few years have created unprecedented opportunities to identify biomarkers of disease processes, especially by using multi-omics technologies and datasets to derive valid and useful signatures of disease. Despite these advances, today only a minority of patients respond to immunotherapies. Prediction of response to therapies such as checkpoint inhibitors that rely on activation of endogenous immune responses has been shown to be especially difficult due to complex and heterogeneous immune escape mechanisms in each patient. Increasing evidence suggests that measurement of robust biomarkers through spatial analysis of the tissue will be key to enable rational patient selection for an improved clinical trial process and design precise combination therapies. The urgency to discover and implement new biomarkers lays bare the need to integrate a variety of advanced tools to probe the dynamic nature of events happening in the tumor microenvironment (TME). Recently, technology updates integrating the use of multiplex immunohistochemistry/immunofluorescence (mIHC/IF) provides much needed insight into cellular composition, cellular functions, and cell-cell interactions. Importantly, recent studies have used mIHC/IF to explore specific immune cells as part of the tumor immune microenvironment (TME) and found that it is helpful for clinical prognosis and efficacy prediction in patients with cancer. In this presentation we will show a streamlined unique workflow supporting whole slide imaging of an 8-plex mIF and traditional same slide H&E fusion on a single tissue slide for a comprehensive tissue immunophenotyping analysis.

Learning Objectives

The utility of a high throughput, high-plex (Immuno-8) staining and mIF assay development for scientists and clinicians

Demonstrate how advanced AI-driven image analysis can be applied to discover cell types, populations and morphological context

Discuss how whole slide image analysis of the tumor microenvironment can provide insight into specific cancer types

Meet the host

Troy is an experienced analytical histotechnologist with expertise in histology, immunohistochemistry, in situ hybridization, digital slide scanning and image analysis within the GLP/GCP and pre-clinical environments along with a strong multidisciplinary CDx and research experience in models of oncology and autoimmune diseases.

Author
Troy Bloom
Senior Field Application Scientist, Ultivue
Categories: 6108 Informing Spatial Biology with Multiplexed Immunofluorescence: A Pathologist’s Perspective
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Sotirios Lakis MD, PhD

Sr. Director Molecular & Computational Pathology, Ultivue

Informing Spatial Biology with Multiplexed Immunofluorescence: A Pathologist’s Perspective

60 minutes

Relevant for

InSituPlex

Description Immunotherapy has transformed the treatment of metastatic and recurrent solid tumors but is challenged in that only a minority of patients respond, especially those therapies that rely on immune activation such as checkpoint inhibitors due to the complex and heterogeneous immune escape mechanisms which can develop in each patient. Therefore, the development of robust biomarkers, enabling rational patient selection and the design of precise combination therapies, is key for the continued success and improvement afforded by this valuable treatment. Overview of what is addressed: Why spatial relationships are important in tumor biology. Examine specific cell clustering, dispersion, and co-localization. To better understand the biology within tissues with a quantitative summary of the regions and phenotypes present.

Webinar Learning Objectives

Enable a better understanding of tumor heterogeneity, its immune environment and contextual relationship using spatial quantification of different immune and tumor cells.

Overview of Spatial Phenomics: Enabling the interrogation of complex cellular phenotypes, functional states, and intercellular relationships with the help of computational biology/pathology methods.

Opportunities and challenges for spatial biology and OMICs applications.

Meet the hosts

As a Senior Director, Molecular and Computational Pathology at UV I am responsible for establishing augmented pathology workflows and for implementing innovative data-driven QC methods. I am a board-certified MD, PhD anatomical pathologist with 10 years of experience in Translational Research, and Cancer Diagnostics.

I am passionate about building cross-disciplinary bridges to deliver innovative services and products while considering the applicable regulatory requirements. I am excited about new ways that molecular and spatial biomarkers can accelerate pharmaceutical research and improve the lives of patients.

Author
Sotirios Lakis MD, PhD

Sr. Director Molecular & Computational Pathology, Ultivue

Categories: 5903 Development of specific multiplexed immunofluorescence immune assays to study mouse models of tumorigenesis
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ebook

Development of specific multiplexed immunofluorescence immune assays to study mouse models of tumorigenesis

Register here for download

Relevant for

Collaboration, InSituPlex, OmniVUE (formerly FixVUE), OmniVUE (formerly FlexVUE)

Description Developing a multiplex immunofluorescence assay for the in depth characterization of activatedT cells in tumor tissue samples.

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5847 Development of specific multiplexed immunofluorescence immune assays to study mouse models of tumorigenesis
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poster

Presented at: AACR 2022

Development of specific multiplexed immunofluorescence immune assays to study mouse models of tumorigenesis

Register here for download

Relevant for

FixVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Authors

Yvette Cajigas, Alina Ainbinder, Gourab Chatterjee, Angela Vasaturo, Holger Weber, Erin Nicola, Mael Manesse, Kirsteen H. Maclean

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5828 High throughput tissue phenotyping and imaging of the tumor immune microenvironment using novel FlexVUE™multiplexed immunofluorescence assays
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poster

Presented at: AACR 2022

High throughput tissue phenotyping and imaging of the tumor immune microenvironment using novel FlexVUE™multiplexed immunofluorescence assays

Register here for download

Relevant for

FlexVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Authors

Yvette Cajigas, Alina Ainbinder, Gourab Chatterjee, Angela Vasaturo, Holger Weber, Erin Nicola, Mael Manesse, Kirsteen H. Maclean

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5829 Improved Understanding of the Biology and Pathophysiology of the TME in PDAC Samples Revealed by Both InSituPlex and Imaging Mass Cytometry
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poster

Presented at: AACR 2022

Improved Understanding of the Biology and Pathophysiology of the TME in PDAC Samples Revealed by Both InSituPlex and Imaging Mass Cytometry

Register here for download

Relevant for

Collaboration, FixVUE, Imaging mass cytometry, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Authors

Yvette Cajigas, Alina Ainbinder, Gourab Chatterjee, Angela Vasaturo, Holger Weber, Erin Nicola, Mael Manesse, Kirsteen H. Maclean

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5814 The power of data analytics in biomarker programs
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Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

The power of data analytics in biomarker programs

Length: 10 minutes

Relevant for

FixVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5796 Introduction to Ultivue’s capabilities for immuno-oncology biomarker programs
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webinar
Moritz Widmaier, PhD; Branden Hopkinson, PhD

Senior Product Manager AI & Data Analytics

Introduction to Ultivue’s capabilities for immuno-oncology biomarker programs

Length: 20 minutes

Relevant for

FlexVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the hosts

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5782 FlexVUE case studies
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Kirsteen MacClean, PhD

Senior Director Scientific Brand Strategy

FlexVUE case studies

Length: 20 minutes

Relevant for

FlexVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5788 Gaining key phenotypic data from the tumor microenvironment using 8-plex immunofluorescence staining and image analysis
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Angela Vasaturo, PhD; Lorcan Sherry, PhD

Associate Director, Scientific Affairs Ultivue; Chief Science Officer, OracleBio

Gaining key phenotypic data from the tumor microenvironment using 8-plex immunofluorescence staining and image analysis

Length: 60 minutes

Relevant for

FixVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Lorcan is an image analysis expert who previously spent 10 years in the pharmaceutical industry as a group leader (Organon, Schering-Plough, Merck & Co.). He led a group responsible for performing histology, immunohistochemistry / image analysis and he has considerable experience in developing translational biomarker strategies for projects across various therapeutic areas. He is a Prince 2® qualified project manager and has extensive experience in study management.

Author
Lorcan Sherry, PhD

Chief Science Officer, OracleBio

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5786 FlexVUE for research
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Bonnie Philips, PhD

Senior Manager, FAS, North America

FlexVUE for research

Length: 20 minutes

Relevant for

FlexVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5808 UltiStacker for mIF
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Doug Wood, PhD

Principal Software Engineer

UltiStacker for mIF

Length: 17 minutes

Relevant for

FixVUE, FlexVUE, InSituPlex, U-VUE

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

Categories: 5784 FlexVUE for biomarker programs
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Angela Vasaturo, PhD

Associate Director Scientific Affairs

FlexVUE for biomarker programs

Length: 20 minutes

Relevant for

FlexVUE, InSituPlex

Description Our poster describes the verification process to development specific pre-clinical mouse specific multiplexed immunofluorescence (mIF) assays. The use of pre-clinical mouse tumor models is widely used to demonstrate efficacy of novel immunotherapies, however a more comprehensive view of their tumor-immune compositions and their relevance to human tumors is needed. The use of specific mouse tissue phenotyping and mIF assays offer the unique advantage of preserving the architectural features of the tumor and revealing the spatial relationships between tumor cells and immune cells.

Meet the host

Moritz will drill his way through whatever intellectual challenge you’ll put in front of him. He previously perforated biochemistry, image analysis, data science, immuno-oncology biomarkers, product management/ownership. He’ll condense the best of our ideas into a coherent roadmap. His product backlog will guide us towards our vision one step at a time.

Author
Moritz Widmaier, PhD

Senior Product Manager AI & Data Analytics

Want to meet with us? Check out our upcoming events.

Want to meet with us? Check out our upcoming events.

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